Modern multicomponent reactions for better drug syntheses

Multicomponent reaction (MCR) technology is now widely recognized for its impact on drug discovery projects and is strongly endorsed by industry as well as academia. However, still relatively few products based on MCRs are marketed or under development. This provides tremendous opportunities for organic chemists to shorten synthetic pathways, thus reducing the cost-of-goods considerably. A recent example of the HCV drug Telaprevir is highlighted where introduction of two MCRs could lead to a shortening of the synthesis route by more than 50%.


Modern multicomponent reactions for better drug syntheses
Tryfon Zarganes-Tzitzikas and Alexander Dömling* Multicomponent reaction (MCR) technology is now widely recognized for its impact on drug discovery projects and is strongly endorsed by industry as well as academia.However, still relatively few products based on MCRs are marketed or under development.This provides tremendous opportunities for organic chemists to shorten synthetic pathways, thus reducing the cost-of-goods considerably.A recent example of the HCV drug Telaprevir is highlighted where introduction of two MCRs could lead to a shortening of the synthesis route by more than 50%.
The ever growing structural complexity of modern drugs has led to an increase in the difficulty of synthetic efforts for their production (Fig. 1).This general trend can be rationalized as more complex proteins and enzymes are emerging as viable drug targets and there is an increasing need for higher selectivity and potency of compounds.
The large scale GMP synthesis of complex drugs is almost automatically reflected in the considerably higher cost-ofgoods.Sometimes, however, a cleverly designed synthetic pathway can lead to rapid, convergent, and cheaper syntheses of otherwise difficult and lengthy-to-access drug targets.An interesting recent case is the 2013 FDA approved hepatitis C NS3 protease inhibitor Telaprevir.
Hepatitis C is a viral infectious disease affecting more than 200 million people worldwide and is currently treated by a combination of PEGylated interferon and ribavirin.However, a significant number of patients do not respond to this therapy due to adverse effects or viral rebound caused by resistant strains.The recent approval of two peptidic HCV NS3 protease inhibitors Telaprevir and Boceprevir has shifted the HCV treatment paradigm and gives patients new and powerful treatment options.The reported technical synthesis of Telaprevir involves

Zarganes-Tzitzikas Tryfon and Alexander Dömling
Zarganes-Tzitzikas Tryfon was born in Thessaloniki, Greece in 1988.He obtained his B.Sc. degree in Chemistry from the Aristotle University of Thessaloniki in 2010.In March 2012 he received his M.Sc.degree with emphasis on Organic Chemistry under the guidance of Professors J. Stephanidou-Stephanatou and K. Tsoleridis from the Aristotle University of Thessaloniki.Since July 2012 he has been pursuing his Ph.D. in Medicinal Chemistry under the guidance of Prof. Alexander Dömling in the Drug Design Group at the University of Gröningen, The Netherlands.
Alexander Dömling has been holding the chair for Drug Design at the University of Groningen since 2011.He studied Chemistry & Biology at the Technische Universität München and obtained his Ph.D. under the guidance of Ivar Ugi.After a postdoc under a Humboldt fellowship in the group of the Nobel Laureate Barry Sharpless he founded the biotech company Morphochem and later Carmolex Inc.After his habilitation he worked as full professor at the University of Pittsburgh in the School of Pharmacy.His interests are centered around multicomponent reaction chemistry (MCR) and its application to problems in drug design.His special focus is on MCR centered pharmacophore methods, structure-baseddrug-design and MCR centered fragment-based-drug-design.He is the author of more than 150 scientific articles, reviews and book contributions.He has applied for more than 30 patents.His long term vision is to bring a novel drug to patients in an indicated area of unmet medical needs.a lengthy, highly linear strategy relying on standard peptide chemistry and entails more than 20 linear steps. 1 For example, the central bicyclic proline derivative is synthesized in racemic form via a nine-step sequence.The desired enantiomer is only obtained after chiral HPLC separation.Optimization of the synthesis of Telaprevir could significantly lower the production costs, thereby making this highly needed drug available to an increased proportion of the world population in the future.
Here we highlight the work of two independent groups towards significant improvements in the synthesis of the HCV drug Telaprevir 1.It has to be noted that both syntheses are currently being investigated for large scale preparation by a pharmaceutical company.Recently Orru and Ruijter et al. from the VU University Amsterdam disclosed a highly efficient and stereoselective synthesis of Telaprevir (Incivek®) employing the combined use of biocatalysts and multicomponent reactions (MCRs).3][4] The significance of the work is a reduction by more than half the steps while comprising a "greener" process which can be additionally translated into a considerably lower cost-of-goods.
A retrosynthetic analysis of 1 involving two MCRs is presented in Fig. 2. The required starting materials for the key Ugi-type 3CR were the carboxylic acid 2, cyclic imine 3, and isocyanide 4. The acid 2 could be accessed by standard peptide chemistry, while the chiral imine 3 was generated in situ from commercially available 5 by the enzyme MAO-N catalyzed oxidation.The isocyanide 4 was accessed via a Passerini threecomponent reaction (P-3CR) of compounds 6 and 7, and acetic acid.
Coupling of pyrazinecarboxylic acid 9 and L-cyclohexylgly- cine methyl ester 10 followed by saponification afforded 11 in excellent yield.Subsequent coupling with L-tert-leucine methyl ester 12 and saponification furnished the required optically pure acid 2. The Orru and Ruijter group was able to significantly increase both the atom and step economy and the overall yield (74% vs. 11% over four steps) of the acid 2 compared to the already known synthesis 1 (Fig. 3).
The construction of the isocyanide fragment 4 was done in three steps in which a Passerini 3-component reaction (P-3CR) played a key role.Commercial (S)-2-amino-1-pentanol 8 was transformed to formamide 13 by N-formylation.Following the work of Ngouansavanh and Zhu, 5 the in situ oxidation of alcohol 13 and a P-3CR was performed in one pot giving access to 14.Both the alcohol oxidation and the Passerini reac-   tion were performed in CH 2 Cl 2 .Thus, one-pot Dess-Martin oxidation/Passerini reaction of 13 furnished 14 in 60% yield.Dehydration then afforded the required isocyanide 4 in very good yield (87%).Importantly, no racemization of the C3 stereocenter in 4 was observed.The diastereomeric stereocenter of 4 in C4 will be removed at a later stage of the synthesis.The crucial building block 4 was thus accessible in only three steps from commercially available starting materials (Fig. 4).
The last step towards the three-component Ugi-type coupling envisaged in the retrosynthesis is described below.7][8] Reacting cyclic Schiff base 3 with the acid 2 and the isocyanide 4 gave the advanced intermediate 15.Finally, cleavage of the acetate followed by Dess-Martin oxidation gave Telaprevir 1 as a 83 : 13 : 4 mixture of diastereomers, with one minor diastereomer derived from the incomplete stereoinduction of the Ugi-type 3CR, and the other from the minor enantiomer of imine 3. Chromatography allowed straightforward separation of the diastereomers to afford pure Telaprevir 1 in 80% yield over the last two steps (Fig. 5).The above synthesis comprises of only eleven steps in total (technical 24), with seven steps in the longest linear sequence and a yield of 45% starting from 10.In conclusion, the MCR approach that the group of Orru and Ruijter proposes gives access to Telaprevir 1 in higher yields and with higher efficiency than any previously disclosed process.Furthermore, the chiral information used for the preparation is derived from readily available and inexpensive building blocks, making the process a highly effective approach to such prolyl dipeptides.
Recently Banfi et al. disclosed an alternative pathway to Telaprevir combining biocatalysis and MCRs. 9,10Their strategy involved the construction of the backbone of the C-terminus of the target compounds while subsequently forming the bicyclic pyrrolidine.Biocatalytic desymmetrization of meso diol 16 followed by a short sequence of standard transformations gives alcohol 17. 11 Next, in situ oxidation of 17 to the aldehyde is done in a Passerini-reaction to afford 18 as a mixture of the diastereoisomer ratio of a/b = 2 : 1.After the azide reduction of 18a, an intramolecular O to N migration occurs to give the amidoalcohol 19.Cyclization via the mesylate 20 proceeded in excellent yields to give 21 (Fig. 6).After deprotection and oxidation, aldehyde 22 is formed.A Passerini reaction takes place in the next step involving compound 22, cyclopropyl isocyanide 24, and acetic acid 25 producing compound 23, which after three steps is converted into Telaprevir.As an additional benefit of the synthesis the volatile cyclopropylisocyanide is not isolated but produced in situ from the formamide precursor.The second route by Banfi et al. although longer than the first one is highlighting alternative synthetic processes which might find use due to intellectual property management and/or better yields and stereoselectivities.
The reduction of the steps of the technical total synthesis of Telaprevir by more than 1/2 using two convergent MCRs in combination with biocatalysis is indeed an intriguing piece of applied chemistry.][14][15][16][17]