Precious metal carborane polymer nanoparticles: characterisation of micellar formulations and anticancer activity.

We report the encapsulation of highly hydrophobic 16-electron organometallic ruthenium and osmium carborane complexes [Ru/Os(p-cymene)(1,2-dicarba-closo-dodecarborane-1,2-dithiolate)] ( and ) in Pluronic® triblock copolymer P123 core-shell micelles. The spherical nanoparticles and , dispersed in water, were characterized by dynamic light scattering (DLS), cryogenic transmission electron microscopy (cryo-TEM), and synchrotron small-angle X-ray scattering (SAXS; diameter ca. 15 and 19 nm, respectively). Complexes and were highly active towards A2780 human ovarian cancer cells (IC(50) 0.17 and 2.50 μM, respectively) and the encapsulated complexes, as and nanoparticles, were less potent (IC(50) 6.69 μM and 117.5 μM, respectively), but more selective towards cancer cells compared to normal cells.


Introduction
Dicarba-closo-dodecarboranes are a class of boron-rich compounds with globular structure and diameter of ca. 1 nm (diameter of a rotating phenyl) that possess unusual properties, including high symmetry and remarkable stability. 1 These 25 characteristics have given rise to numerous applications, and carboranes have been used as building blocks in various systems, such as dendrimers, 2 polymers, 3 and nanoparticles. 4,5 They also have been extensively studied as potential boron neutron capture (BNCT) therapeutic agents, 6,7 and as bioisosters of phenyl groups and pharmacophores for targeted drug development. 8 Nevertheless, selective and 30 effective delivery of boron agents is still a key issue which hinders their further clinical development. 9 On the other hand, half-sandwich complexes of ruthenium and osmium are a versatile class of organometallic compounds. Their biological properties have raised considerable expectations for the treatment of cancer since the beginning of the 2000s and are considered as a promising alternative to platinum- 35 based chemotherapeutics. 10,11 We have recently discussed how the combination of arene ruthenium(II)/osmium(II) complexes and carboranes has unexplored potential in medicine. 12 Furthermore, such complexes exhibit unusual chemistry: coordination of the bulky, electron-deficient carborane ligand 1,2-dicarba-closo-dodecarborane-1,2-dithiolato to an arene-Ru or Os metal centre leads to the isolation of rare stable 1,2-dithiolate)] (1 and 2). However, these complexes being highly hydrophobic, their biological applications are impaired by the lack of solubility in water. We have recently discussed how nanotechnology may help to overcome such challenges in medicinal inorganic chemistry. 13 Here, we report the encapsulation of these hydrophobic complexes in water-soluble polymer micelles. 5 The class of ABA triblock copolymers, where A = hydrophilic block poly(ethylene oxide) (PEO) and B = hydrophobic block poly(propylene oxide) (PPO), are commercially available as Pluronic ® (non-proprietary name "poloxamers") and offer a pool of more than 50 amphiphilic, 14 water-soluble and polymorphic materials. The physical and chemical properties of Pluronic ® copolymers can be finely tuned by 10 modifying the molar mass ratio between the PEO and PPO blocks (from 1: 9 to 8:2), which directly modifies the in vivo properties and interactions with cells and cell membranes, and provides high potential for the design of innovative nanomedicines and new biomaterials. 15 To exploit the chemistry of carborane-containing arene ruthenium complexes in aqueous solution, and to take advantage of their unique 15 properties, we have encapsulated the 16-electron complexes 1 and 2 in Pluronic ® triblock copolymer P123 micelles (Fig. 1). The resulting polymeric micelles RuMs and OsMs, dispersed in water, have been characterized by UV-visible spectroscopy, dynamic light scattering (DLS), cryogenic-transmission electron microscopy (cryo-TEM), and synchrotron small-angle X-ray scattering (SAXS) studies. 20 Complexes 1 and 2 and micelles RuMs and OsMs were tested against the A2780 human ovarian cancer cell line. The ruthenium-based complex and micelles were also tested against MRC5 fibroblasts, as an example of non-cancerous but fast dividing cells to determine their selectivity and potential therapeutic window. Such nanotechnology-based strategy not only allows the utilisation of precious metal 25 complexes containing carborane ligands in aqueous solution, but also offers the possibility of formulating and delivering a large number of these complexes (ca. 60 per particle) to cancer cells, thus generating a high intracellular boron concentration (ca. 600 per particle). There is much current interest in the therapeutic use of boronbased pharmaceuticals, owing to their chemico-biological properties, to expand 30 medicinal potential in prevention, diagnosis and therapy. 16

Characterisation of the micellar formulations RuMs and OsMs
The organometallic half-sandwich Ru II and Os II arene complexes [Ru/Os(pcymene)(1,2-dicarba-closo-dodecarborane-1,2-dithiolate)] (1/2) were synthesized as reported previously. 17 These complexes have a pseudo-octahedral structure, with a 35 π-bonded arene occupying 3 coordination sites, a S-bound chelated dithiolato dicarba-closo-dodecarborane ligand, and a vacant 6 th site (Fig. 1). They are 16electron complexes and therefore electron-deficient at the metal. 18 Complexes 1 and 2 are highly hydrophobic and insoluble in water. 8 To achieve dispersion in water, 13 we encapsulated 1 and 2 in the water-soluble amphiphilic triblock copolymer P123 40 (poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)) (PEO-PPO-PEO), by mixing a tetrahydrofuran (THF) solution of 1 or 2 with an aqueous solution of polymer P123 (THF/H 2 O: 1/10 v/v; [complex] = 5mg/mL), at ambient temperature for 4 h (Fig. 1). The encapsulation of the metal complexes in P123 micelles (P123Ms) was accompanied by a dramatic colour change from transparent to blue and purple for RuMs and OsMs, respectively, indicative of the presence of the 16-electron complexes 1 and 2 in the micelles. We have recently reported the full characterisation of OsMs micelles, 19 and now present the 19.6±1.8 nm to 11.9±4.1 nm for RuMs and 11.5±2.3 nm for OsMs with dispersities (Ð) of 0.04 and 0.03, respectively. Although micellar size usually increases after encapsulation of organic molecules, incorporation of hydrophobic molecules can result in expulsion of water from micelles, causing a contraction . 20 The hydrophobicity of 1 and 2 probably results in a stronger folding of the PPO chains 30 around the complexes through hydrophobic interactions, with concomitant expulsion of water from the core. A small-in-number (<0.01 % in number) second population of RuMs and OsMs particles is found at D h ~ 220 nm, exhibiting a strong intensity in DLS, and is due to the aggregation of some particles. Cryogenic transmission electron microscopy (cryo-TEM) analysis without staining was performed on Quantifoil ® carbon coated grids so as to observe the morphology of the nanoparticles in solution. The high contrast provided by the presence of the heavy ruthenium and osmium centres allowed facile imaging without staining (Fig.   5 3). From these experiments, it was clear that spherical micellar morphologies are formed when polymer P123 encapsulates complexes 1 or 2. The observed diameters of these nano-spheres are 10.45±3.43 nm for RuMs and 7.85±1.97 nm for OsMs with very low dispersities (<1.12). These data are in accordance with the hydrodynamic diameters determined by DLS, within experimental error. To gain further insight into their structures in aqueous solution, and to confirm cryo-TEM and DLS results, RuMs, OsMs and P123Ms were analyzed by synchrotron small-angle X-ray scattering (SAXS; Fig. 4). The experimental profiles were fitted 35 using IgorPro software 21 to three model functions for spherical micelles: SphereForm, 22 CoreShellSphere, 22 and PolyCoreShellRatio 23 (PCR). The PCR model fitted excellently for all micelles with very low dispersity parameters (0.124 for RuMs, 0.161 for OsMs and 0.146 for P123Ms, 0 being an ideal mono-disperse system, Table 1). These analyses demonstrated that RuMs and OsMs self-assembly 40 leads to core/shell micelles with a core diameter of 9.88±0.25 nm and 9.06±0.12 nm, and a shell diameter of 9.09±0.29 nm and 6.50±0.15 nm, respectively (Table 1). The diameters of RuMs and OsMs micelles by DLS and cryo-TEM are in accordance with the core diameter determined by SAXS within the experimental errors. From this data (scattering length density calculations, degrees of polymerization of 45 Pluronic ® P123, and the molecular formulae of the polymer and of complex 1/2), aggregation numbers for RuMs, OsMs and P123Ms micelles were determined as 20±2 monomer chains per P123Ms micelle, 66±4 monomer chains per RuMs micelles and 52±6 monomer chains per OsMs micelles. Determinations of ruthenium and osmium by inductively coupled plasma mass spectrometry (ICP -MS) gave a polymer/complex 1 ratio of 1/0.9±0.11 for RuMs and 1/1±0.09 for OsMs showing that the 66 polymer chains self-assembled with 59±14 complexes 1 in RuMs and 52 monomer chains self-assembled with 52±11 complexes 2 (see Table  1).

Anticancer activity of complexes 1 and 2 and micellar formulations RuMs and OsMs
Synthetic polymer therapeutics are of particular interest in medicine, due to their 25 synthetic versatility, as well as their tunable properties. 24 A number of biologically active polymer-drug conjugates and polymeric formulations, such as micelles, hydrogels and polymer-coated nanoparticles, are currently in clinical development. 25 Among the most commonly used polymers for applications in medicine are the ABA Pluronic ® triblock block copolymers, which are particularly suitable for the design 30 of bio-inspired, bioengineered and biomimetic polymer nanoparticles. The utilisation of Pluronic ® block copolymers as drug delivery systems, 26-31 biological response modifiers, 32-36 pharmaceutical ingredients, 27,37,38 and steric stabilizers to lyotropic liquid crystalline particles, [39][40][41] has led to recent clinical advances. 15 Inorganic compounds offer different mechanisms of drug action depending on the metal used, their structures and redox properties. 11, Thus, they can be utilized for the design of novel drugs in the treatment of a broad range of diseases, 86 and their combination with nanotechnology tools such as polymer nanoparticles may 5 provide opportunities for tackling medical challenges in the near future. 13 Surprisingly, only a few studies have been devoted to the encapsulation of inorganic compounds (mainly cisplatin) in Pluronic ® block copolymer-based nanostructures. 87, 88 10 We studied the antiproliferative activity of complexes 1, 2 and micelles RuMs and OsMs in A2780 human ovarian cancer cells ( Table 2). Cells were exposed for 24 h to the complexes (dissolved in 5% dimethyl sulfoxide (dmso)/95% saline and further diluted in cell culture medium until working concentrations in the range of 100 M-0.01M were achieved with a maximum dmso concentration of 1%) or micelles 15 (100% saline, further diluted with cell culture medium to a range of concentration 200M-0.10M). After this, drugs were removed and cell were washed and placed in fresh growth medium for a further 72 h as a recovery period. Cell viability was then assessed using the sulforhodamine B (SRB) colorimetric assay. Complex 1 is highly potent (IC 50 170 nM), about 7× more potent than the clinical drug cisplatin. It 20 is also 39× more potent than RuMs micelles, which still exhibit good (micromolar) activity towards cancer cells, and is as potent as the clinical drug carboplatin. Interestingly, RuMs micelles are 4× more selective than 1, showing only moderate toxicity towards MRC5-fibroblast cells. Finally, the formulation of complexes 1 and 2 in polymer micelles has allowed dispersal of hydrophobic ruthenium and osmium 25 arene complexes in water in a manner suitable for administration to cancer cells (administration to cancer cells in water, without the need to add dmso).

Conclusion
We have encapsulated highly hydrophobic carborane-containing precious-metal Faraday Discussions complexes in triblock copolymer micelles. This has allowed dispersal of hydrophobic ruthenium and osmium arene complexes in water in a manner suitable for administration to cancer cells. Although entrapment of the 16-electron complexes 1 and 2 in Pluronic ® micelles leads to a reduction in their anticancer potency, the micelles exhibit enhanced selectivity towards cancer cells compared to 5 normal cells. Polymer encapsulation of metal carborane complexes also provides the potential for delivering high amounts of boron to cells which is of interest for boron neutron capture therapy (BNCT). The neutron capture ability of such micelles is currently under investigation.

Experimental section
10

Materials
The preparation of the complexes [Ru/Os(p-cym)(1,2-dicarba-closo-dodecaborane-1,2-dithiolato)] (1 and 2) was based on a previous report. 17 The preparation of the OsMs micelles was based on a previous report. 19 The triblock copolymer P123 15 [poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol)] was purchased from Sigma-Aldrich and used as received. Anhydrous tetrahydrofuran (Aldrich) was used. 18.2 mega-ohm purity water was collected from a Purelab ® UHQ USF Elga system. Holey carbon grids with 200 mesh and lacey carbon grids were purchased from Quantifoil Micro Tools Gmbh and Elektron 20 Technology UK Ltd, respectively and used as received.

Synthesis of RuMs
A tetrahydrofuran (THF) solution (1 mL) of complex 1 (5 mg/mL) was added to an 25 aqueous solution (10 mL) of polymer P123 (5 mg/mL) and the resultant mixture was stirred at ambient temperature for 4 h. The solution was then dialyzed to remove the THF (MWCO = 1000 Da), for 48 h, and then freeze-dried. A similar procedure was used for synthesizing OsRuMs with 1 mol equiv of 1, 1 mol equiv of the Ru analogue and 1 mol equiv of polymer P123. 30 Instrumentation UV-visible spectroscopy: UV-visible absorption spectra were recorded on a temperature-controlled Varian CARY 300 Biospectrophotometer using 1-cm path- 35 length quartz cuvettes (0.5 mL).
Inductively coupled plasma mass spectrometry: Osmium or ruthenium content was determined using an ICP-MS Agilent technologies 7500 series instrument. The standard for osmium was purchased from Aldrich. Calibration curves were prepared 40 using Ru/Os standard solutions in double deionised water (ddw) with 3% nitric acid, ranging between 50 and 0.5 ppb (9 points). Samples were freshly prepared in ddw with 3% nitric acid. Readings were made in no-gas mode with a detection limit of 1 ppt. 45 Dynamic Boltzmann constant, T is the temperature and η is the viscosity of the solvent. D h coincides with the hydrodynamic diameter when the sample is made of monodispersed spherical particles (D app equals the translational diffusion D t ).
Cryogenic electron microscopy cryo-TEM: A JEOL 2010F TEM was operated at 200 10 keV and images were recorded on a Gatan UltraScan 4000 camera for cryo -TEM and glow discharge. The samples were prepared at ambient temperature by placing a droplet on a TEM grid. The extra liquid was then blotted with a filter paper and the grid was inserted in liquid ethane at its freezing point. The frozen samples were subsequently kept under liquid nitrogen. 15 Small-angle X-ray scattering (SAXS): Measurements were carried out on the SAXS beamline at the Australian Synchrotron facility at a photon energy of 11 keV. The samples in solution were in 1.5 mm diameter quartz capillaries. The measurements were collected at a sample to detector distance of 3.252 m to give a q range of 0.004 20 to 0.2 Å -1 , where q is the scattering vector and is related to the scattering angle (θ) and the photon wavelength (λ) by the following equation (1): The scattering from a blank (H 2 O) was measured in the same location as sample collection and was subtracted for each measurement. Data were normalized for total transmitted flux using a quantitative beamstop detector and absolute scaled using water as an absolute intensity standard. The two-dimensional SAXS images were converted in one-dimensional SAXS profiles (I(q) versus q) by circular averaging, 30 where I(q) is the scattering intensity. Functions were used from the NCNR package. Scattering length densities were calculated using the "Scattering Length Density Calculator" provided by NIST Center for Neutron Research.
Cell culture. A2780 human ovarian carcinoma and MRC5 human fetal lung 35 fibroblasts were obtained from the European Collection of Cell Cultures (ECACC). Both cell lines were grown in Roswell Park Memorial Institute medium (RPMI -1640) supplemented with 10% of fetal calf serum, 1% of 2 mM glutamine and 1% penicillin/streptomycin. All cells were grown as adherent monolayers at 310 K in a 5% CO 2 humidified atmosphere and passaged at approximately 70-80% confluency. 40 In vitro growth inhibition assay. Briefly, 96-well plates were used to seed 5000 cells per well. The plates were left to pre-incubate in drug-free media at 310 K for 48 h before adding different concentrations of the compounds to be tested. A drug exposure period of 24 h was allowed. After this, supernatants were removed by 45 suction and each well was washed with PBS. A further 48 h was allowed for the cells to recover in drug-free medium at 310 K. The SRB assay was used to determine cell viability. IC 50 values, as the concentration which caused 50% of cell death, were determined as duplicates of triplicates in two independent sets of