Issue 18, 2014

Metalloprotein design using genetic code expansion

Abstract

More than one third of all proteins are metalloproteins. They catalyze important reactions such as photosynthesis, nitrogen fixation and CO2 reduction. Metalloproteins such as the olfactory receptors also serve as highly elaborate sensors. Here we review recent developments in functional metalloprotein design using the genetic code expansion approach. We show that, through the site-specific incorporation of metal-chelating unnatural amino acids (UAAs), proton and electron transfer mediators, and UAAs bearing bioorthogonal reaction groups, small soluble proteins can recapitulate and expand the important functions of complex metalloproteins. Further developments along this route may result in cell factories and live-cell sensors with unprecedented efficiency and selectivity.

Graphical abstract: Metalloprotein design using genetic code expansion

Article information

Article type
Tutorial Review
Submitted
14 Jan 2014
First published
04 Apr 2014

Chem. Soc. Rev., 2014,43, 6498-6510

Author version available

Metalloprotein design using genetic code expansion

C. Hu, S. I. Chan, E. B. Sawyer, Y. Yu and J. Wang, Chem. Soc. Rev., 2014, 43, 6498 DOI: 10.1039/C4CS00018H

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