Neocuproine-functionalized silica-coated magnetic nanoparticles for extraction of copper(II) from aqueous solution silica-coated extraction copper(II) from Neocuproine-functionalized silica-coated magnetic nanoparticles for extraction of copper( II ) from aqueous solution †‡

Neocuproine has been covalently bound to silica-coated maghemite ( c -Fe 2 O 3 ) magnetic nanoparticles (MNPs) by a phenyl ether linkage. The resulting MNPs are able to remove Cu( II ) from 12 ppm aqueous solution with an extraction eﬃciency of up to 99% at pH 2. Magnetic nanoparticles (MNPs) combine high surface area with ease of separation 1 and the use of iron oxide particles has opened fascinating separation applications. 2–7 However, iron oxide based MNPs in combination with complexing agents cannot be used in acidic media, which would dissolve the particles. 8 A way to solve this problem is to use silica to provide a chemically unreactive surface whilst not affecting the core. 8 Furthermore, the free Si–OH surface groups can allow effective covalent binding of organic functional groups onto the surface of the SiO 2 -coated MNPs. Commonly, surface modification with alkoxysilanes of the general formula X–(CH 2 ) n –Si(OR) 3 , is used where X represents the head-group functionality, (CH 2 ) n that acts as a flexible spacer, and Si(OR) 3 the anchor group that can attach to the free Si–OH groups on the surface of the MNPs. The work combines the stability of SiO 2 -coated MNPs and the complexing power of neocuproine 11 in order to study the capabilities of such materials for the effective removal of Cu( II ) from aqueous solutions at different pH.

3][4][5][6][7] However, iron oxide based MNPs in combination with complexing agents cannot be used in acidic media, which would dissolve the particles. 8A way to solve this problem is to use silica to provide a chemically unreactive surface whilst not affecting the core. 8urthermore, the free Si-OH surface groups can allow effective covalent binding of organic functional groups onto the surface of the SiO 2 -coated MNPs.Commonly, surface modification with alkoxysilanes of the general formula X-(CH 2 ) n -Si(OR) 3 , is used where X represents the head-group functionality, (CH 2 ) n that acts as a flexible spacer, and Si(OR) 3 the anchor group that can attach to the free Si-OH groups on the surface of the MNPs. 9,10he present work combines the stability of SiO 2 -coated MNPs and the complexing power of neocuproine 11 in order to study the capabilities of such materials for the effective removal of Cu(II) from aqueous solutions at different pH.
In order to introduce functionality at the 5-position of neocuproine 4, bromination 15,16 was carried out with bromine (0.6 equivalents) in the presence of fuming H 2 SO 4 (20% SO 3 ).Replacement of the bromine with a 4-hydroxyphenol linking group was successfully achieved via Suzuki coupling 17 with 4-hydroxyphenylboronic acid (Scheme 2).This phenol functionalized ligand could then be immobilized onto the MNPs by nucleophilic substitution of the iodo-substituent (Scheme 3).
Each functionalization step of the MNPs was followed by infrared spectroscopy.The unfunctionalized g-Fe 2 O 3 MNPs 1 were characterized by X-ray powder diffraction 18 and were found to consist of pure maghemite or g-Fe 2 O 3 ; 19 whereas diffraction data of the composite nanoparticles after modification with silica did not show any well defined peaks (ESI ‡), indicating that the silica coating is thick enough to interfere with the iron oxide diffraction, or the iron oxide is more amorphous than before (smaller particles, smaller diffracting domains or more disordered structure) allowing the silica pattern to dominate.
Representative TEM images of g-Fe 2 O 3 MNPs 1, iodoalkylfunctionalized SiO 2 -coated MNPs 3 and neocuproinefunctionalized SiO 2 -coated MNPs 7, shown in Fig. 3, were recorded on the particles dispersed in methanol and dried on a copper grid at room temperature.Fig. 3(a) shows the predominantly spherical morphology of the g-Fe 2 O 3 MNPs 1 with an average diameter of ca. 15 nm.These tended to form aggregates as shown in Fig. 3(b).In the case of iodoalkyl-functionalized SiO 2 -coated MNPs 3 [Fig.3(c)], the diameter of the particles was found to be ca.20-25 nm, while Fig. 3(d) reveals that neocuproine-functionalized SiO 2coated MNPs 7 have diameter of ca.50-55 nm.
To complement the TEM images that only provide information on the size of the MNP cores, DLS measurements (ESI ‡) were also carried out.This indicated the g-Fe 2 O 3 MNPs 1, iodoalkylfunctionalized SiO 2 -coated MNPs 3 and neocuproine-functionalized SiO 2 -coated MNPs 7 to have narrow particle size distributions and average diameter values of 15, 48, and 72 nm, respectively.The values measured for the iodoalkyl-functionalized SiO 2 -coated MNPs 3 and neocuproine-functionalized SiO 2 -coated MNPs 7 are larger compared to the TEM images; this phenomenon has been observed previously. 20,21he organic content on the MNPs was investigated using thermal gravimetric analysis (TGA) under nitrogen.The weight loss for the SiO 2 -coated MNPs 2 was about 2.5% over the temperature range from 60-200 1C, presumably due to the loss of residual water; whereas the TGA curve of iodoalkylfunctionalized SiO 2 -coated MNPs 3 showed a sharp weight loss    at 250-300 1C, proposed to correspond to the loss of iodoalkyl coating (ESI ‡).The TGA curve (Fig. 4) of neocuproinefunctionalized SiO 2 -coated MNPs 7 shows three weight-loss steps.Below 200 1C, the weight loss is quite small, probably resulting from the removal of absorbed water.After that, there is a significant weight loss from 300-500 1C corresponding to the decomposition of the organic components.From this, it can be estimated that the amount of neocuproine bound onto the MNPs is about B15% w/w (ESI ‡).Further weight loss at 700-900 1C can be attributed to the formation of iron carbide. 22,23he extraction of Cu(II) from aqueous media was tested by weighing about 12 mg of functionalized MNPs 7 into plastic tubes containing 10 mL solutions of 12 ppm of Cu(II) at different pH.The mixtures were sonicated 5 min and then shaken overnight.A neodymium permanent magnet was placed for 60 seconds beneath the tube to move the MNPs to the tube wall allowing the supernatant liquid to be decanted.After separation, the supernatant was subjected to quantitative elemental analysis by atomic absorption spectrometry.
Acidity of an aqueous solution is known to exert a profound influence on extraction efficiency of various ligands. 5The effect of solution pH on Cu(II) extraction was investigated in the range pH 2-8.As shown in Fig. 5, close to 99% of Cu(II) was removed at pH 2 (initial concentration 12.33 ppm, final concentration 0.13 ppm); although the extraction efficiency decreased with increasing the pH.The lowest pH was then selected for kinetic studies where extraction was effectively complete after 5 min (ESI ‡).
The percentage of Cu(II) extraction from a 12 ppm initial concentration with different amounts of neocuproinefunctionalized MNPs 7 at pH 2 is presented in Fig. 6 1).
The extraction efficiencies of SiO 2 -coated Fe 2 O 3 MNPs 2 and iodoalkyl-functionalized SiO 2 -coated MNPs 3 were found to be 17% and 30%, respectively; compared to the 99% extraction efficiency observed for neocuproine functionalized MNPs 7. Thus, the majority of the extraction capacity of functionalized MNPs 7 is due to the bound neocuproine.
In summary, neocuproine functionalized MNPs 7 have been prepared and characterised.These MNPs have the advantages of high surface area and their ability to be separated from aqueous media magnetically.Their ability to extract Cu(II) from aqueous media has been investigated at varying pH, with the maximum extraction efficiency of Cu(II) by the MNPs being found to be 99% at pH 2. Extraction was complete within 5 min and about 12 mg of these MNPs were able to alter the concentration of Cu(II) in 10 mL of solution from 12.33 ppm to 0.13 ppm.
The authors acknowledge the EPSRC for financial support (A.A.).Use of the Chemical Analysis Facility (CAF) and Centre for Advanced Microscopy (CfAM) at the University of Reading is gratefully acknowledged.We also would like to thank Mr Michael Andrews, Dr Peter Harris and Miss Anne Dudley for their assistance with X-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Atomic Absorption (AA) measurements, respectively.

Notes and references
Neocuproine has been covalently bound to silica-coated maghemite (c-Fe 2 O 3 ) magnetic nanoparticles (MNPs) by a phenyl ether linkage.The resulting MNPs are able to remove Cu(II) from 12 ppm aqueous solution with an extraction efficiency of up to 99% at pH 2.

Fig. 1
depicts the FT-IR spectra of uncoated (g-Fe 2 O 3 ) MNPs 1, SiO 2 -coated MNPs 2 and iodoalkylfunctionalized SiO 2 -coated MNPs 3. The g-Fe 2 O 3 MNPs 1 cause two strong absorptions at 630 and 580 cm À1 .The introduction of silica on the surface of the g-Fe 2 O 3 MNPs 1 results in an additional absorption band at 1080 cm À1 owing to Si-O stretching.After functionalization with 3-iodopropyltrimethoxysilane, bands at 2930 cm À1 and 688 cm À1 were observed, assigned to the C-H stretching and C-I stretching modes of iodoalkyl-functionalized SiO 2 -coated MNPs 3, respectively.The FT-IR spectra shown in Fig. 2 demonstrate a clear distinction between iodoalkyl-functionalized SiO 2 -coated MNPs 3 and neocuproine-functionalized SiO 2 -coated MNPs 7. Absence of the C-I stretching absorption at 688 cm À1 and presence of bands at 1500-1600 cm À1 owing to CQC aromatic vibrations are indicative of the covalent incorporation of neocuproine onto the MNPs.
and shows an almost linear correlation of Cu(II) extraction with the amount of MNPs 7 added.To assess the chelating effect of the bound neocuproine in functionalized MNPs 7, the extraction efficiencies of SiO 2 -coated Fe 2 O 3 MNPs 2 and iodoalkyl-functionalized SiO 2 -coated MNPs 3 were also investigated at pH 2 and compared with the extraction efficiency of neocuproine functionalized MNPs 7 (Table

Fig. 5
Fig. 5 Effect of initial pH on the extraction efficiency of Cu(II) by MNPs 7.