Pyrrolidone Modified Magnetic COF Materials for The Quantitative Determination of 2,5-hydroxyvitamin D in Human Serum via Isotope Dilution Mass Spectrometry

Abstract

Vitamin D is indispensable in regulating the balance of calcium and phosphate and preserving the health of the skeletal system. Rapid and acurate detection of vitamin D content in the blood is crucial. In this work, we synthesized a magnetic COF material modified with pyrrolidone and p-aminobenzoic acid (Fe3O4@COF@Au@PABA@APP) for quantifying 2,5hydroxyvitamin D from serum via isotope dilution mass spectrometry. The developed method demonstrated excellent linearity (R 2 > 0.99) across the 1-200 ng/mL calibration range, low detection limit (0.50 ng/mL), good recovery rate (97.69%-103.16%), and low differences in intra-day (< 6.04%) and inter-day (< 7.50%). The measurement uncertainty for 2,5-OH VD2 and VD3 were determined to be about 3.46% and 3.60%, respectively. The VD in the serum of 20 volunteers was successfully measured by this method, which proved that the method had remarkable reliability and repeatability.