Membrane destabilization by monomeric hIAPP observed by Imaging Fluorescence Correlation Spectroscopy

Membrane destabilization by monomeric hIAPP observed by Imaging Fluorescence Correlation Spectroscopy Nirmalya Bag, Ashraf Ali, Virander Singh Chauhan, Thorsten Wohland* and Aseem Mishra* Departments of Biological Sciences and Chemistry and NUS Centre for Bio-Imaging Sciences (CBIS), National University of Singapore, Singapore 117557 Malaria Research Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India 110067

Membrane association of aggregation-prone proteins and peptides has emerged as a critical factor in numerous degenerative diseases.In the last two decades, human Islet Amyloid Polypeptide (hIAPP) was shown to be an important amyloidogenic peptide implicated in progressive pancreatic degeneration in diabetes mellitus type II.hIAPP is a 37-residue peptide hormone that is co-packaged and co-secreted with insulin upon glucose stimulus and has been found to regulate physiological processes viz.gastric emptying and regulation of insulin response. 1Of particular interest is the observation that in vitro aggregation of hIAPP causes membrane damage by a 'pore formation' mechanism that is believed to cause cytotoxicity. 2A concern in these assays, however, is the high concentration of hIAPP used (10-40 mM) at which it aggregates; while physiological hIAPP exists as a monomer at a concentration of 20-100 pM. 1 Thus, it is imperative to investigate the mode of interaction of monomeric hIAPP and its effect on membranes in an attempt to discover the identity of the toxic entity in the case of pancreatic degeneration.
In this work, we used membrane dynamics as a metric to probe monomeric hIAPP induced membrane perturbation.We explored the effect of monomeric hIAPP on the fluidity of different supported lipid bilayers (SLBs) and live cell membranes using single molecule sensitive imaging total internal reflection-fluorescence correlation spectroscopy (ITIR-FCS) 3,4 and provide a mechanism of the hIAPPmembrane interaction.The detailed methods are given in the ESI.† We first establish that hIAPP exists as a monomer at 1 mM and lower concentrations by a variety of methods.We used confocal FCS to measure the diffusion time (t D ) of Atto-R6G labelled hIAPP (AR6G-hIAPP).FCS can distinguish monomers and aggregates based on their respective diffusion times.The t D of AR6G-hIAPP was 66 AE 0.6 ms, which corresponds to the diffusion coefficient (D) = 311 AE 5 mm 2 s À1 at concentrations of 40 nM and 1 mM (blue and green autocorrelation functions (ACFs) in Fig. 1A respectively).The obtained value of D is in good agreement with the previous reports for monomeric hIAPP as measured by confocal FCS and diffusion-NMR experiments. 5,6one of the temporal intensity traces of 10 independent measurements on AR6G-hIAPP at each of these concentrations showed any intensity spikes supporting the absence of aggregates (Fig. 1B).However, intensity traces of 40 mM hIAPP contain spikes indicative of aggregates (Fig. 1C).The existence of aggregates is confirmed by the right shift of the ACF as aggregates diffuse much slower than the monomeric species (Fig. 1A, red).Next, the TEM image of 1 mM hIAPP did not show any feature (Fig. 1D, top left).For comparison, TEM images of oligomers (top right), protofibrils (bottom left) and fibrils (bottom right) of hIAPP are shown in Fig. 1D.A novel penta-thiophene dye, pFTAA, that selectively binds to amyloidogenic oligomers 7 did not show any increase in its fluorescence intensity (Fig. 1E) nor a change in its absorption spectra (Fig. S1A, ESI †) upon addition of 1 mM unlabeled hIAPP suggesting that hIAPP did not form oligomers.The conventional thioflavin T assay also did not show any fibril formation at this concentration (Fig. S1B, ESI †).The CD spectra of hIAPP at 1 mM concentration (Fig. S1C 2A, green).For the same concentration of peptide, an SLB composed of DOPC : DPPC : Chol (1 : 1 : 1), a canonical 'raft mixture', exhibited a 70% increase in D (Fig. 2A, red).An SLB composed of DOPC : Chol (5 : 1), however, showed only 40% increase in D (Fig. 2A, blue) whereas hIAPP had little effect on a pure DOPC SLB (Fig. 2A, black).We also observed an almost instantaneous formation of fluorescent structures in the different bilayers upon addition of the peptide (Video 1, ESI †).As these structures were brightly fluorescent, we infer that they contain a large number of lipid molecules extracted from the RhoPE labelled SLB along with the non-labeled peptide as a non-specific hetero-complex.Though a similar process has been proposed earlier, we present experimental evidence that the extraction of lipid affects its stability. 8The observed increase in membrane fluidity by lipid extraction is inversely correlated to the 'free area' of the membrane (DOPC > DOPC : Cholesterol > DOPC : DPPC : Chol > DOPC : DPPC).Lipid extraction from a gel lipid containing SLBs causes much larger release of free area compared to that of fluid SLBs and thus a dramatic change in D was observed for DOPC : DPPC SLBs.
The increase in membrane fluidity was also dependent on the peptide concentration used (Fig. 2B).Low concentration of unlabelled hIAPP (50 nM) did not cause any change in D of the RhoPE labelled DOPC : DPPC (1 : 1) bilayer (3.0 AE 0.4 mm 2 s À1 at 310 K) suggesting that physiological levels of hIAPP would be non-damaging to cellular membranes.However, at moderately higher concentrations (200-500 nM) we found that the peptide increased fluidity.Higher peptide concentrations (1 mM) resulted in a still higher increase in membrane fluidity (D = 5.8 AE 1.2 mm 2 s À1 ).Though intuitive, we demonstrate here that the extraction of lipids from the bilayer by sub-micromolar hIAPP concentrations results directly in the increase in membrane fluidity and not necessarily collapse.
Nevertheless, hIAPP has to interact with the SLB to be able to extract lipid.To probe the association of the peptide with the SLB, we added non-damaging concentration of AR6G-hIAPP (50 nM) to an unlabelled SLB and imaged the system using total internal reflection fluorescence (TIRF) microscopy.TIRF videography showed the peptide to form a diffusing carpet-like structure over the membrane (Fig. 2C and Video 2, ESI †).The dark areas in the image (and in Video 2, ESI †) are areas of membrane defect/collapse where AR6G-hIAPP does not form a carpet.The apparent diffusion coefficient of the peptide was computed to be 9.03 AE 5.92 mm 2 s À1 , which was roughly 3 times higher than the free diffusing lipid probe in the membrane suggesting no penetration of hIAPP (Fig. S2, ESI †).Recent studies indicate that hIAPP can assume betasheet-like structures on artificial surfaces without forming higher order oligomers/fibres. 9Moreover, the predictions from the Monte Carlo simulation (using the MCpep server 10 ) suggest that the insertion of hIAPP into the membrane is not energetically favourable (Fig. 2D and Fig. S3, ESI †).hIAPP assumes an amphiphilic helical structure with two short hydrophobic stretches alternating with polar patches (Fig. S4, ESI †), 11 a conformation that may not favour its penetration into the membrane but facilitate surface orientation (as predicted) favoured by local electrostatics as in ALPS/class A2 domains. 12onomeric hIAPP did not form pores in liposomes as observed from the confocal FCS based dye leakage experiments (the principle of the experiment is given in Fig. S5, ESI †).The dye was not released from either rhodamine labelled vesicles (RLVs) or rhodamine entrapping vesicles (RLVs) upon treatment with 1 mM of unlabelled monomeric hIAPP (Fig. S6A and B, ESI †), rather the liposomes aggregated.As expected, hIAPP at aggregating concentration (40 mM) caused dye release (Fig. S7, ESI †).We therefore believe that monomeric hIAPP associates with the membrane following the 'carpet model' instead of the widely speculated 'pore model'. 13e next explored if hIAPP interacts with live cell membranes in a similar way.DiI-C 18 labelled SH-SY5Y membranes in the absence of hIAPP showed a D of 2.4 AE 0.4 mm 2 s À1 at 310 K (Fig. 3A).Unlabelled hIAPP (1 mM) was added to the cells, as this concentration was sufficient to increase membrane fluidity for a SLB.A time dependent increase in membrane fluidity (D = 4.1 AE 1.2 mm 2 s À1 ) in the initial 20 min was observed (Fig. 3A).Thereafter, brightly fluorescent plasma membrane derived structures were visible that exhibited an apparently slower D (Fig. 3B and C).The standard deviation (SD) is a reflection of cell membrane heterogeneity and is in a typical range of 17-25% as seen by FCS measurements for the cell membrane. 14For measurements at later time points, the SD rises to 95% indicating an increase in heterogeneity after hIAPP interaction (see ESI † for details).Although difficult to ascertain, these structures might resemble vacuole like structures formed in b cells upon hIAPP treatment that are associated with cellular degeneration. 15In our experiment, the number and size of these structures increased with time.We show a 'FCS movie' indicating temporal changes in spatial distribution of D at a region of interest (ROI) with the described bright structures (Video 3, ESI †).The principle of the 'FCS movie' is given in Fig. S8 (ESI †).
To understand the diffusion behaviour of the abovedescribed structures, we utilized the 'FCS diffusion law' analysis implemented on ITIR-FCS that identifies confined diffusion from a plot of diffusion time versus the observation area. 16tails of the analysis are given in the ESI.† It was interesting to observe that the FCS diffusion law intercept remained close to zero until 15 min while the fluidity of the membrane increased during this time (Fig. 3D).Beyond this time, an increase in the intercept is associated with an overall decrease in D. The positive intercept indicates the formation of non-penetrable domains.This correlated well with the appearance of the bright clusters in the image sequence.Cytotoxicity assay using MTT also indicated cell death (B80%) after 14 h of incubation with 1 mM hIAPP suggesting that the observed membrane destabilization is cytotoxic (Fig. S9, ESI †).These observations suggest that the increase in fluidity of the plasma membrane by hIAPP induces a quantifiable biological response in the form of membrane reorganization not observed in artificial bilayers.Thus from the SLB and live cell membrane studies, we conclude that monomeric hIAPP interacts with membranes via dynamic carpet formation followed by lipid extraction in a concentration dependent manner (Fig. 3E).
Our results suggest that monomeric hIAPP, alone, can enhance membrane fluidity depending on its concentration and the membrane composition and also of the live plasma membranes leading to significant membrane reorganization.Based on these findings, we propose that monomeric hIAPP can present itself as a cytotoxic entity beyond a critical concentration; pre-aggregation per se may not be essential.However, we cannot exclude the possibility that hIAPP forms oligomers or aggregates during or after its interaction with the membrane.Nonetheless, amyloid plaques are implicated in degenerating pancreas of diabetes mellitus type II patients.We believe that our findings will prove valuable in the understanding of hIAPP mediated cellular damage in a new light and aid in the design of relevant therapeutic interventions.

Fig. 1
Fig. 1 Aggregation state of hIAPP.(A) ACFs of AR6G-hIAPP at different concentrations.(B) Intensity traces of 40 nM (blue) and 1 mM (green) AR6G-hIAPP show no spike indicating the absence of aggregates.(C) Intensity trace of 40 mM (red) AR6G-hIAPP shows a number of spikes indicating the presence of aggregates (the inset shows the same trace with a magnified y-axis showing a wide range of different intensity peaks).(D) TEM images of monomeric (1 mM) hIAPP (top left) and the oligomers (top right), protofibrils (bottom left) and fibrils (bottom right) of hIAPP.(E) The fluorescence spectra of the aggregation sensing dye pFTAA in the absence (blue open square) and in the presence of 1 mM (red close square) and 40 mM (black triangle) unlabelled hIAPP.
, ESI, † red) exhibited a dominant helical component suggesting the absence of fibrils.At 40 mM hIAPP aggregated into amyloid fibres (Fig. S1D, ESI †) with b-sheet conformation (Fig. S1C, ESI, † black).Taken together, these observations suggest that hIAPP remains monomeric in the range of concentrations (40 nM-1 mM) used for this study.Interaction of unlabelled hIAPP with the rhodamine PE (RhoPE) labelled SLBs was studied by measuring the lateral diffusion coefficient (D) of the latter at 310 K. Membrane diffusion is a potent reporter of its integrity.The D of the DOPC : DPPC (1 : 1) SLB which exhibits gel/fluid phase co-existence increased in a time dependent manner.The D increased from 3.0 AE 0.4 mm 2 s À1 to 5.8 AE 1.2 mm 2 s À1 (at 40 min) (Fig.

Fig. 2
Fig. 2 Interaction of monomeric hIAPP with SLBs.(A) Percentage increase in D of different RhoPE labelled SLBs upon treatment with unlabelled hIAPP (1 mM).(B) hIAPP concentration dependent increase in D of the RhoPE labelled DOPC : DPPC (1 : 1) bilayer.(C) ACFs of the diffusing 'carpet' of AR6G-hIAPP (blue, corresponds to the bright region of the TIRF image shown in the inset) and background (black, corresponds to the dark region of the same image).In the inset, the TIRF image shows that AR6G-hIAPP carpets only form on the unlabelled DOPC SLB top (bright), not on the non-membrane region (dark).(D) The predicted orientation of hIAPP over a membrane by MCPep shows peptide insertion to be unfavorable.

Fig. 3
Fig. 3 Interaction of monomeric hIAPP with plasma membranes.(A) A time dependent increase and subsequent decrease in D of DiI labeled plasma membrane on treatment with 1 mM unlabelled hIAPP.(B) TIRF images of the lower membrane of hIAPP treated cells (at measured ROI) at different time points.The scale bar is 1 mm.(C) ACFs of the labelled membrane inside and outside the bright structures.(D) Time dependent increase in FCS diffusion law intercept (t 0 ).The inset shows the diffusion law plots at representative time points.(E) Schematic representation of 'carpeting' and lipid extraction by monomeric hIAPP.