Panwajee Payongsri, David Steadman, John Strafford, Andrew MacMurray, Helen C. Hailes and Paul A. Dalby
Org. Biomol. Chem., 2012,10, 9021-9029
DOI:
10.1039/C2OB25751C,
Paper
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.
