Magnetic nanoparticles for the affinity adsorption of maltose binding protein (MBP) fusion enzymes

Abstract

By the combination of fusion protein technology and nanoparticle surface modification technology, a maltose-modified magnetic support has been successfully developed for the integration of separation/purification, and immobilization of maltose binding protein–Heparinase I fusion enzyme (MBP–HepA) for enzymatic reaction and recycle. HepA is an important enzyme to degrade heparin and heparan sulfate to produce low molecular weight heparin (LMWH). This magnetic support may play an important role for process simplification and cost reduction on the industrial production of LMWH. Further, this magnetic support also can be used for the immobilization of other MBP fusion proteins, which implies that this magnetic support may have wide applications in biotechnology and enzyme engineering.