A label-free dual-modal aptasensor for colorimetric and fluorescent detection of sulfadiazine

Abstract

Sulfadiazine (SDZ) residues in food products and the environment pose a serious threat to human health and ecological balance, thereby warranting the development of new methods for simple, rapid and accurate detection of these compounds. To this end, we developed a novel label-free dual-modal aptasensor for SDZ detection based on distance-dependent color change of gold nanoparticles (AuNPs) and fluorescence resonance energy transfer between AuNPs and rhodamine B (RhoB). In this aptasensor, the binding of the aptamer to SDZ causes unprotected AuNPs to aggregate in NaCl solution, which alters the color of the solution and restores the fluorescence of RhoB. Under optimal conditions, the aptasensor exhibited a linear colorimetric response in the SDZ concentration range of 50–1000 ng mL⁻¹, and a linear fluorescence response in the SDZ concentration range of 4–256 ng mL⁻¹. The limits of detection for colorimetric and fluorescent readings were 28 ng mL⁻¹ and 2 ng mL⁻¹ respectively. The recoveries of SDZ in the spiked real samples were 88.28–108.44% by colorimetry and 90.27–106.04% by fluorometry. Furthermore, the results of this aptasensor showed excellent correlation (R² ≥ 0.9858) with HPLC findings. Taken together, these experimental results demonstrate that the proposed label-free dual-modal aptasensor can be employed to screen for SDZ contamination in food and environmental samples.