Issue 14, 2021

A compact fiber-integrated optofluidic platform for highly specific microRNA Förster resonance energy transfer detection

Abstract

MicroRNAs (miRNAs) have attracted extensive interest as promising biomarkers for the profiling of diseases. However, quantitative measurement of miRNAs presents a significant challenge in biochemical studies. In this work, we developed an innovative optofluidic platform to perform a rapid, simple, quantitative and high-specificity miRNA assay using the Förster resonance energy transfer (FRET) principle. A novel three-way junction FRET probe was proposed to enable rapid and enzyme-free miRNA detection. Using this platform, we performed one-step, amplification-free miRNA detection with simple device operation and achieved miRNA identification at a low concentration. The detection system could achieve high specificity for discrimination of three-base mismatches, and the sample volume was significantly reduced, favorable for low-level miRNA detection in material-limited samples. The establishment of a compact, low-cost, highly sensitive and selective miRNA analysis platform provides a valuable tool for point-of-care diagnosis.

Graphical abstract: A compact fiber-integrated optofluidic platform for highly specific microRNA Förster resonance energy transfer detection

Supplementary files

Article information

Article type
Paper
Submitted
23 Feb 2021
Accepted
16 Apr 2021
First published
20 Apr 2021

Analyst, 2021,146, 4454-4460

A compact fiber-integrated optofluidic platform for highly specific microRNA Förster resonance energy transfer detection

H. Feng, L. Liu, Y. Chen, W. Shu, Y. Huang, B. Zhang, T. Wu, Z. Jin and Y. Chen, Analyst, 2021, 146, 4454 DOI: 10.1039/D1AN00324K

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