RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African Swine Fever Virus DNA
Abstract
An RAA-CRISPR-HCR (RCH) cascade amplification system was developed, which generates an ultrasensitive colorimetric response to low concentrations of African Swine Fever Virus (ASFV) DNA with a detection limit of 1 copy/µL. It demonstrates excellent accuracy and specificity in real samples, facilitating efficient on-site visual early detection of ASFV.
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