Issue 7, 2005
From the journal:

Chemical Communications

Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism†

Yoichiro Ijima,a   Kaori Matoishi,a   Yosuke Terao,a   Nobuhide Doi,a   Hiroshi Yanagawaa  and  Hiromichi Ohta*a  

* Corresponding authors

a Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
E-mail: hohta@bio.keio.ac.jp
Fax: +81-(0)45-566-1551
Tel: +81-(0)45-566-1703

Abstract

The introduction of two mutations (G74C/C188S) based on the estimated reaction mechanism resulted in the inversion of enantioselectivity of arylmalonate decarboxylase, which catalyses the asymmetric decarboxylation of arylmethylmalonate to give optically active arylpropionate.

Graphical abstract: Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

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Article information

DOI
https://doi.org/10.1039/B416398B
Article type
Communication
Submitted
25 Oct 2004
Accepted
19 Nov 2004
First published
10 Jan 2005

Chem. Commun., 2005, 877-879

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Inversion of enantioselectivity of asymmetric biocatalytic decarboxylation by site-directed mutagenesis based on the reaction mechanism

Y. Ijima, K. Matoishi, Y. Terao, N. Doi, H. Yanagawa and H. Ohta, Chem. Commun., 2005, 877 DOI: 10.1039/B416398B

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