Cationic porphyrin-based nanoparticles for photodynamic inactivation and identification of bacteria strains

Abstract

The emergence of antibiotic drug resistance has undermined the efficacy of antibiotics, and is becoming a severe threat to public health. To combat antibiotic drug resistance and to replace traditional antibiotic treatment, an alternative strategy based on antibacterial photodynamic therapy (APDT), which has broad applicability, high efficiency and less potential of developing antibiotic drug resistance, has been developed. In this work, the cationic porphyrin-based nanoparticles (NPs) were prepared by epoxy–amine chain extension polymerization of diepoxy-terminated poly(ethylene glycol) (PEG) and tetraamino-containing porphyrin, followed by quaternization with methyl iodine and butyl bromide. The as-obtained cationic porphyrin NPs preserved the photophysical properties of porphyrin derivatives, and can efficiently generate singlet oxygen (¹O₂) under 635 nm laser irradiation. The cationic porphyrin-based NPs displayed intrinsic antibacterial properties, and exhibited strong APDT effect on Gram-positive bacteria by destroying the bacterial cell membranes. Upon incubation with different bacterial strains, it was found that they could be utilized to identify Gram-positive bacteria by observing the sedimentation behavior of their mixtures, and visualizing their co-cultured and centrifugal bacteria cakes. In addition, the cationic porphyrin-based NPs had good hemocompatibility and low dark cytotoxicity.