Issue 6, 2010

Micro-scale process development of transaminase catalysed reactions

Abstract

A micro-scale technique has been developed for the process development of transaminase catalysed reactions. This pH indicator based, colorimetric assay can be used to investigate and optimise reaction conditions at 100 μL scale. Enzyme activity and stability as a function of various reaction parameters, including temperature, pH and co-solvent concentration, have been determined. Additionally, reactions have been scaled up from 100 μL to 25 mL under the optimal reaction conditions identified by the micro-scale process development activities. Excellent conversion (>99%) and enantioselectivity (>99% ee) were obtained.

Graphical abstract: Micro-scale process development of transaminase catalysed reactions

Supplementary files

Article information

Article type
Communication
Submitted
19 Nov 2009
Accepted
11 Jan 2010
First published
22 Jan 2010

Org. Biomol. Chem., 2010,8, 1280-1283

Micro-scale process development of transaminase catalysed reactions

M. D. Truppo and N. J. Turner, Org. Biomol. Chem., 2010, 8, 1280 DOI: 10.1039/B924209K

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