Measurement of the different affinities of the two halves of glycopeptide dimers for acetate

Abstract

The titration of sodium acetate into solutions of the vancomycin group antibiotics eremomycin and chloroeremomycin has been studied by observing the downfield movement of the ¹ H NMR signals due to amide protons in the antibiotic binding pocket; this change in chemical shift has been used to determine the different affinities of the two halves of the dimers for acetate ligand.