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Issue 4, 2020

Single-molecule nanopore sensing of actin dynamics and drug binding

Author affiliations

Abstract

Actin is a key protein in the dynamic processes within the eukaryotic cell. To date, methods exploring the molecular state of actin are limited to insights gained from structural approaches, providing a snapshot of protein folding, or methods that require chemical modifications compromising actin monomer thermostability. Nanopore sensing permits label-free investigation of native proteins and is ideally suited to study proteins such as actin that require specialised buffers and cofactors. Using nanopores, we determined the state of actin at the macromolecular level (filamentous or globular) and in its monomeric form bound to inhibitors. We revealed urea-dependent and voltage-dependent transitional states and observed the unfolding process within which sub-populations of transient actin oligomers are visible. We detected, in real-time, filament-growth, and drug-binding at the single-molecule level demonstrating the promise of nanopore sensing for in-depth understanding of protein folding landscapes and for drug discovery.

Graphical abstract: Single-molecule nanopore sensing of actin dynamics and drug binding

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Supplementary files

Article information


Submitted
11 Nov 2019
Accepted
02 Dec 2019
First published
03 Dec 2019

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2020,11, 970-979
Article type
Edge Article

Single-molecule nanopore sensing of actin dynamics and drug binding

X. Wang, M. D. Wilkinson, X. Lin, R. Ren, K. R. Willison, A. P. Ivanov, J. Baum and J. B. Edel, Chem. Sci., 2020, 11, 970 DOI: 10.1039/C9SC05710B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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