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Issue 1, 2020
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Evaluation of curcumin-mediated photodynamic therapy on the reverse of multidrug resistance in tumor cells

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Abstract

Curcumin (CUR) possesses photosensitive anti-tumor activity. However, photoactive CUR mainly targets tumor cells sensitive to chemotherapy, whereas the effect on multi-drug resistant cancer cells has not been fully investigated. The study aimed to investigate the anti-tumor activity of CUR on resistant MCF-7/ADM cells and its underlying mechanism providing insights into CUR-mediated PDT and a reference for reversing multidrug resistance. Cell apoptosis and morphological changes were detected by Annexin V-FITC/PI double staining and immunofluorescence, respectively. The apoptosis mechanism of CUR-mediated PDT was investigated by detecting the levels of reactive oxygen species (ROS), mitochondrial membrane potential, and related proteins. MTT and apoptosis results showed that CUR-mediated PDT significantly enhanced cytotoxicity and induced considerable cell apoptosis. After treatment with CUR-mediated PDT, cells became round in shape and shrunk, F-actin was loosely arranged, and the nucleus decreased in size. In addition, the level of ROS increased over time compared to the control and peaked at 6 h. CUR-mediated PDT induced alterations in the mitochondrial membrane potential, increased the release of mitochondrial cytochrome C (Cyt-c), and downregulated caspase-3/7/9, PARP, and P-gp. In conclusion, CUR-PDT induced apoptosis in resistant MCF-7/ADM cells primarily through endogenous mitochondrial apoptosis pathway. Besides apoptosis activation in resistant cells, the reverse of multidrug resistance was ascribed to the downregulation of P-gp expression to a degree.

Graphical abstract: Evaluation of curcumin-mediated photodynamic therapy on the reverse of multidrug resistance in tumor cells

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Supplementary files

Article information


Submitted
29 Nov 2019
Accepted
17 Dec 2019
First published
02 Jan 2020

This article is Open Access

RSC Adv., 2020,10, 298-306
Article type
Paper

Evaluation of curcumin-mediated photodynamic therapy on the reverse of multidrug resistance in tumor cells

Y. Li, P. Xiao, Z. Huang, X. Chen, X. Yan, J. Zhai and Y. Ma, RSC Adv., 2020, 10, 298
DOI: 10.1039/C9RA09996D

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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