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Issue 22, 2012
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Programmable microfluidic synthesis of spectrally encoded microspheres

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Abstract

Spectrally encoded fluorescent beads are an attractive platform for assay miniaturization and multiplexing in the biological sciences. Here, we synthesize hydrophilic PEG–acrylate polymer beads encoded with lanthanide nanophosphors using a fully automated microfluidic synthesis device. These beads are encoded by including varying amounts of two lanthanide nanophosphors relative to a third reference nanophosphor to generate 24 distinct ratios. These codes differ by less than 3% from their target values and can be distinguished from each other with an error rate of <0.1%. The encoded bead synthesis strategy we have used is readily extensible to larger numbers of codes, potentially up to millions, providing a new platform technology for assay multiplexing.

Graphical abstract: Programmable microfluidic synthesis of spectrally encoded microspheres

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Article information


Submitted
20 Jun 2012
Accepted
21 Sep 2012
First published
25 Sep 2012

This article is Open Access

Lab Chip, 2012,12, 4716-4723
Article type
Paper

Programmable microfluidic synthesis of spectrally encoded microspheres

R. E. Gerver, R. Gómez-Sjöberg, B. C. Baxter, K. S. Thorn, P. M. Fordyce, C. A. Diaz-Botia, B. A. Helms and J. L. DeRisi, Lab Chip, 2012, 12, 4716
DOI: 10.1039/C2LC40699C

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