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Volume 187, 2016

Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications

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Abstract

Several proof-of-concept studies on the vibrational spectroscopy of biofluids have demonstrated that the methodology has promising potential as a clinical diagnostic tool. However, these studies also show that there is a lack of a standardised protocol in sample handling and preparation prior to spectroscopic analysis. One of the most important sources of analytical errors is the pre-analytical phase. For the technique to be translated into clinics, it is clear that a very strict protocol needs to be established for such biological samples. This study focuses on some of the aspects of the pre-analytical phase in the development of the high-throughput Fourier Transform Infrared (FTIR) spectroscopy of some of the most common biofluids such as serum, plasma and bile. Pre-analytical considerations that can impact either the samples (solvents, anti-coagulants, freeze–thaw cycles…) and/or spectroscopic analysis (sample preparation such as drying, deposit methods, volumes, substrates, operators dependence…) and consequently the quality and the reproducibility of spectral data will be discussed in this report.

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Supplementary files

Article information


Submitted
19 Nov 2015
Accepted
10 Dec 2015
First published
14 Dec 2015

Faraday Discuss., 2016,187, 521-537
Article type
Paper

Biofluid infrared spectro-diagnostics: pre-analytical considerations for clinical applications

L. Lovergne, P. Bouzy, V. Untereiner, R. Garnotel, M. J. Baker, G. Thiéfin and G. D. Sockalingum, Faraday Discuss., 2016, 187, 521 DOI: 10.1039/C5FD00184F

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