A hybrid membrane-camouflaged CuS nanoplatform for synergistic nanotherapy of ovarian cancer via chemodynamic and sonodynamic effects

Abstract

Cell membrane camouflage technology has been widely used as a key strategy to overcome the limitations of traditional synthetic nanoparticles in terms of blood circulation, immune clearance, and tumor accumulation. In this study, a hybrid membrane-coated CuS nanoplatform (CuS–BSA–PpIX@SR) modified with red blood cell membranes (RBCMs) and SKOV3 cancer cell membranes (CCMs) was constructed for targeted chemodynamic and sonodynamic synergistic therapy of ovarian cancer. The prepared CuS–BSA–PpIX@SR nanoplatform possessed a uniform spherical morphology, excellent dispersibility, and high structural stability. Moreover, the nanoplatform generated abundant ROS under ultrasound irradiation, effectively inducing oxidative stress and apoptosis in SKOV3 cells with enhanced sonodynamic activation efficiency. Furthermore, this nanosystem exhibits excellent tumor accumulation capacity, a longer circulating half-life, and significant tumor-suppressing effects in vivo, with its efficacy attributed to the synergistic effect of chemodynamics and sonodynamics. The CuS–BSA–PpIX@SR nanoplatform integrates multiple functions, including biomimetic membrane-mediated targeted delivery, effective activation of sonosensitizers, and enhancement of oxidative stress responses, achieving highly efficient, precise, and low-toxicity sonodynamic therapy (SDT) in an ovarian cancer model. This strategy not only provides innovative design ideas and technical support for sonodynamic therapy of deep tumors but also opens a new path for the translation of multifunctional biomimetic nanosystems into clinical precision oncology treatment.