Innovative design of fluorescent PLGA–1,8-naphthalimide nanoparticles as multifunctional materials for next-generation nanotechnology and biomedicine

Yuriev Danil; Tkachenko Sergey; Ermolin Danila; Ivanov Ilya; Melnikov Pavel; Malinovskaya Julia; Ryabova Anastasia; Mishin Alexander; Perfilov Maxim; Ramil Khasbiullin; Medvedev Michael; Skorb Ekaterina; Oshchepkov Maxim; Gelperina Svetlana; Oshchepkov Alexander

doi:10.1039/D5TB02866C

Innovative design of fluorescent PLGA–1,8-naphthalimide nanoparticles as multifunctional materials for next-generation nanotechnology and biomedicine

Yuriev Danil,

^a Tkachenko Sergey,

^a Ermolin Danila,

^b Ivanov Ilya,

^bk Melnikov Pavel,

^c Malinovskaya Julia,

^a Ryabova Anastasia,

^def Mishin Alexander,

^g Perfilov Maxim,

^g Ramil Khasbiullin,

^h Medvedev Michael,

^bk Skorb Ekaterina,

^b Oshchepkov Maxim,

^a Gelperina Svetlana

^a and Oshchepkov Alexander

*^ij

Author affiliations

* Corresponding authors

^a Mendeleev University of Chemical Technology of Russia, Miusskaya pl., 9, Moscow, Russian Federation

^b Infochemistry Scientific Center, ITMO University, 9 Lomonosova Str., St. Petersburg, Russian Federation

^c Rudolf Virchow Center, Center for Integrative and Translational Bioimaging, University of Wuerzburg, Josef-Schneider-Str. 2, Würzburg, Germany

^d Prokhorov General Physics Institute of the Russian Academy of Sciences, Vavilov Str. 38, Moscow, Russian Federation

^e National Research Nuclear University MEPHI, Kashirskoye Highway 31, Moscow, Russian Federation

^f RUDN University, Miklukho-Maklaya str. 6, Moscow, Russian Federation

^g Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS (IBCh RAS), Miklukho-Maklaya 16/10, Moscow, Russian Federation

^h Frumkin Institute of Physical Chemistry and Electrochemistry Russian Academy of Sciences (IPCE RAS), 31-4, Leninsky prospect, Moscow, Russian Federation

ⁱ Martin Luther University Halle-Wittenberg, Kurt-Mothes-Straße 2, D-06120 Halle, Germany
E-mail: alexander.oshchepkov@chemie.uni-halle.de

^j Max Planck Institute for the Science of Light, Department of Physics, D-91058 Erlangen, Germany

^k N.D. Zelinsky Institute of Organic Chemistry of Russian Academy of Sciences, 119991 Moscow, Russian Federation

Abstract

Poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles featuring covalently incorporated 1,8-naphthalimide fluorophores were developed through DFT/TD-DFT-guided molecular engineering. Systematic variation of 4-position substituents and carbon spacer lengths established clear structure–property relationships governing spectral–luminescent characteristics and conjugate stability. Computational modeling accurately predicted experimental absorption/emission features. The optimized PLGA-fluorophore conjugates yielded nanoparticles with high aqueous fluorescence, excellent colloidal stability, and approximately two-fold higher photostability than PLGA–Cy5 under the tested imaging conditions. Live-cell confocal microscopy (405 nm excitation) demonstrated strong emission and homogeneous distribution in 4T1/HeLa cells, confirming suitability for in vitro cellular imaging with high cell viability. This covalent PLGA labeling platform establishes a quantifiable foundation for advanced fluorescent nanomaterials in cellular imaging applications. While these results establish a robust platform for cellular imaging applications, extended in vivo validation remains a goal for future work.

Journal of Materials Chemistry B

Innovative design of fluorescent PLGA–1,8-naphthalimide nanoparticles as multifunctional materials for next-generation nanotechnology and biomedicine

Abstract

Supplementary files

Article information

Download Citation

Permissions

Innovative design of fluorescent PLGA–1,8-naphthalimide nanoparticles as multifunctional materials for next-generation nanotechnology and biomedicine

Social activity

Search articles by author

Spotlight

Advertisements