Electrochemically-generated ferricyanide enables thiol–ene capture of protein–protein binding

Abstract

Due to its efficiency, selectivity and biocompatibility, the thiol–ene coupling has demonstrated its potential for protein labelling. Here, we report the electrochemical thiol–ene activation to capture protein–protein binding events. In situ electrochemical generation of Fe(III) was used as the first example of an electrochemically generated thiol–ene protein–protein labelling reaction.