Dual-biocatalytic L-lactate production from gaseous CO2 and acetaldehyde

Abstract

L-Lactate has attracted considerable attention as a precursor for biodegradable plastics, poly L-lactic acid (PLA). It is expected that producing biodegradable plastic precursors from CO₂ and bio-based materials will lead to sustainable plastic use, recovery, and recycling. In this study, a new method for the production of L-lactate via pyruvate as an intermediate from acetaldehyde and gaseous CO₂ in the presence of thiamine pyrophosphate (TPP) and nicotinamide adenine dinucleotide reduced form (NADH) was developed by dual-biocatalytic system of pyruvate decarboxylase (PDC; Enzyme Commission numbers (EC) 4.1.1.1) from Lactobacillus YK1 and L-lactate dehydrogenase (LDH; EC 1.1.1.27) from chicken heart without the toxic raw material such as a hydrogen cyanide. By using dual-biocatalytic system of PDC and LDH, 0.35% of the acetaldehyde was successfully converted to L-lactate after 5 h incubation.