Two New Prenylated Tryptophan Analogues Isolated from the Coral-derived Fungus Tritirachium sp. SCSIO 41037

Abstract

Two new prenylated tryptophan analogues, tritianamides A (1) and B (2), together with ten known nitrogen-containing compounds (3-12), were isolated from the ethyl acetate extract of coral-derived fungus Tritirachium sp. SCSIO 41037. The compounds were purified by extraction, silica gel column chromatography and semi-preparative HPLC. Their structures were identified using NMR and HR-ESI-MS data. The absolute configurations of 1 and 2 were determined by the calculated ECD.In bioassay, all compounds were screened for enzyme inhibitory activity against acetylcholinesterase (AChE) in both virtual screening and in vitro assays. The results revealed that 3 and 5 exhibited moderate inhibitory effects, with IC50 values of 0.32 and 0.46 mM, respectively. We also propose a biosynthetic pathway of 1 and 2 originating from tryptophan.