Multi-stage assembled Lactobacillus reuteri-capsule patch for persistent anti-infection

Abstract

Recurrent bacterial invasion delayed the recovery of infected site so that provide one sustained antibacterial therapy strategy is essential. Here, we used one simple multi-stage assembly strategy to developed a Lactobacillus reuteri-capsule patch that could achieve sustained antibacterial applications. The encapsulated strain in sodium alginate (SA) capsule effectively maintained their activity and exhibited the sustained release behavior through responding to pH-change of infection microenvironment. In addition, the nanofiber structure of bacteria cellulose (BC) shielded the contact between strains and lesion to avoid additional inflammatory reactions (isolation rate reached 99.9%). This patch shown the sustained antibacterial capacity against the formation of common pathogen`s biofilm by hinder the biosynthesis (including E. coli, S. aureus, MRSA and P. aeruginosa), which avoid recurrent bacterial invasion. Transcriptomics results also demonstrates that this patch shown various bioactivities to promote the proliferation, migration and vascularization, in contrast with traditional antibiotic therapy. In vivo infection model verification further demonstrates that this patch effectively clearance biofilm to prevent the recurrent invasion of P. aeruginosa, while achieving substantial tissue regeneration through collagen deposition and neovascularization. This strategy provides a simple and efficient approach for persistent anti-infection application.