Sweet optimization: glucose-vitrified samples for hyperpolarizing glutamine in biological studies

Abstract

This work describes the development of a versatile formulation using glucose as a vitrification agent in dynamic nuclear polarization. Significantly high polarization levels are achieved through optimization of a sample formulation, using sodium acetate and glutamine as targets. The best optimized formulation allowed precise monitoring of the enzymatic conversion of glutamine to glutamate, paving the way for advances in cellular metabolism applications.