Ensemble and single particle analysis of doxorubicin silk nanoparticles

Abstract

Adsorption-loaded silk nanoparticles exploit protein electrostatics and β-sheet-rich architecture to achieve high doxorubicin loading and pH-responsive release, while preserving silk structure. Ensemble and single-particle analyses reveal exceptional formulation uniformity, while in vitro studies confirm preserved cytotoxicity against MDA-MB-231 human breast cancer cells.