G/T Mismatch Discrimination by positioning the mismatch site close to the catalytic core of a 10-23 DNAzyme sensor

Abstract

Single nucleotide variation (SNV) detection is crucial for diagnosis of several diseases. A 10-23 binary DNAzyme-based sensor was able to differentiate the challenging G→A substitution with an initial differentiation factor of >83%, which rose to >98% after further optimization, when the SNV site was positioned close to the DNAzyme catalytic core, introducing an SNV discrimination principle not solely governed by duplex thermodynamics.