No-wash Immunosensors Based on Superhydrophilic Covalent Organic Framework Nanosheets with Aggregation-Induced Emission for Detecting Carbohydrate Antigen 19-9

Abstract

How to enhance the aggregation-induced emission (AIE) activity and hydrophilicity of AIE-covalent organic framework nanosheets (CONs) is an important challenge in improving the sensitivity of sandwich-type fluorescence-on immunosensors. This work developed a 2,2',2'',2'''-(ethene-1,1,2,2-tetrayltetrakis([1,1'-biphenyl]-4',4-diyl))tetraacetonitrile-based AIE-CONs containing cyano and hydroxyl groups with high AIE activity and hydrophilicity to construct highly sensitive immunosensor for detecting carbohydrate antigen 19-9.