Targeted Detection Method for Locus-specific m⁶A Modifications in Low-Abundance Transcripts Based on Chemical Conversion

Abstract

In this study, we developed a method for locus-specific m⁶A detection, which employs sodium nitrite and glyoxal-based chemical conversion with probe hybridization. This approach enables highly sensitive detection of target m⁶A sites even in lowabundance transcripts.

Supplementary files

Article information

Article type
Communication
Submitted
24 Jan 2026
Accepted
17 Mar 2026
First published
18 Mar 2026
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2026, Accepted Manuscript

Targeted Detection Method for Locus-specific m⁶A Modifications in Low-Abundance Transcripts Based on Chemical Conversion

T. Liu, X. Fang, Y. Lu, X. Liu, R. Zhao, X. Zhou and X. Weng, Chem. Commun., 2026, Accepted Manuscript , DOI: 10.1039/D6CC00494F

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