From the journal:

Chemical Communications

Targeted detection method for locus-specific m6A modifications in low-abundance transcripts based on chemical conversion

Ting Liu,†a   Xin Fang,†a   Yue Lu,a   Xiumin Liu,a   Ruiqi Zhao,*a   Xiang Zhou ORCID logo ab  and  Xiaocheng Weng ORCID logo *ab  

* Corresponding authors

a College of Chemistry and Molecular Sciences, State Key Laboratory of Metabolism and Regulation in Complex Organisms, Wuhan University, Wuhan, Hubei, China
E-mail: rqzhao@whu.edu.cn, xcweng@whu.edu.cn

b Wuhan TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, Hubei, P. R. China

Abstract

In this study, we developed a method for locus-specific m6A detection, which employs sodium nitrite and glyoxal-based chemical conversion with probe hybridization. This approach enables highly sensitive detection of target m6A sites even in low-abundance transcripts.

Graphical abstract: Targeted detection method for locus-specific m6A modifications in low-abundance transcripts based on chemical conversion

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Article information

DOI
https://doi.org/10.1039/D6CC00494F
Article type
Communication
Submitted
24 Jan 2026
Accepted
17 Mar 2026
First published
18 Mar 2026
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2026, Advance Article

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Targeted detection method for locus-specific m6A modifications in low-abundance transcripts based on chemical conversion

T. Liu, X. Fang, Y. Lu, X. Liu, R. Zhao, X. Zhou and X. Weng, Chem. Commun., 2026, Advance Article , DOI: 10.1039/D6CC00494F

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