Targeted Detection Method for Locus-specific m⁶A Modifications in Low-Abundance Transcripts Based on Chemical Conversion
Abstract
In this study, we developed a method for locus-specific m⁶A detection, which employs sodium nitrite and glyoxal-based chemical conversion with probe hybridization. This approach enables highly sensitive detection of target m⁶A sites even in lowabundance transcripts.
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