A LEGO-Inspired Multipiece Chip for Portable Nucleic Acid Detection

Abstract

We report a LEGO-Inspired Multipiece chip (LIM-Chip), a modular microfluidic system capable of streamlining multiple nucleic acid amplification and detection steps through on-demand chip assembly via universal 3D-printed side release buckle connectors. The clinical applicability of the LIM-Chip is demonstrated by mediating a DNAzyme-based isothermal nucleic acid amplification assay for the sensitive detection of HPV16 DNA in clinical cervical samples.