RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African Swine Fever Virus DNA

Abstract

An RAA-CRISPR-HCR (RCH) cascade amplification system was developed, which generates an ultrasensitive colorimetric response to low concentrations of African Swine Fever Virus (ASFV) DNA with a detection limit of 1 copy/µL. It demonstrates excellent accuracy and specificity in real samples, facilitating efficient on-site visual early detection of ASFV.

Supplementary files

Article information

Article type
Communication
Submitted
07 Dec 2025
Accepted
27 Jan 2026
First published
30 Jan 2026

Chem. Commun., 2026, Accepted Manuscript

RAA-CRISPR-HCR cascade amplification for ultrasensitive visual detection of African Swine Fever Virus DNA

H. Wu, S. Lin, X. Zuo, W. Hua, T. Sun, G. Shao, F. Li, D. Zhao, Z. Feng, D. Zhu and L. Wang, Chem. Commun., 2026, Accepted Manuscript , DOI: 10.1039/D5CC06815K

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