From the journal:

Chemical Communications

Potassium ion fluorescent sensing in chemically stimulated mammalian cells using a GINKO2-encapsulated sensor fibremat

Azumi Sako,a   Yusuke Nasu,bcd   Akiko Obata ORCID logo a  and  Toshihisa Mizuno ORCID logo *ae  

* Corresponding authors

a Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya, Aichi 466-8555, Japan
E-mail: toshitcm@nitech.ac.jp

b Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan

c Institute of Biochemical Sciences, National Taiwan University, Da’an, Taipei 106, Taiwan

d Department of Chemistry, School of Science, The University of Tokyo, Bunkyo-ku, Tokyo, Japan

e Department of Nanopharmaceutical Sciences, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-cho Showa-ku, Nagoya, Aichi 466-8555, Japan

Abstract

The nanofibre interior of electrospun fibremats provides an effective platform for immobilising sensor proteins for biomolecule detection. We developed fibremats with GINKO2—a fluorescent K+ sensor—encapsulated in nanofibres. This method enabled reversible K+ detection even with protease and real-time monitoring of changes in the K+ concentration secreted by chemically stimulated mammalian cells.

Graphical abstract: Potassium ion fluorescent sensing in chemically stimulated mammalian cells using a GINKO2-encapsulated sensor fibremat

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Article information

DOI
https://doi.org/10.1039/D5CC05871F
Article type
Communication
Submitted
15 Oct 2025
Accepted
27 Jan 2026
First published
30 Jan 2026

Chem. Commun., 2026, Advance Article

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Potassium ion fluorescent sensing in chemically stimulated mammalian cells using a GINKO2-encapsulated sensor fibremat

A. Sako, Y. Nasu, A. Obata and T. Mizuno, Chem. Commun., 2026, Advance Article , DOI: 10.1039/D5CC05871F

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