A novel CRISPR/Cas14a-synergized wood-based platform for ultrasensitive detection of aflatoxin B1

Abstract

A CRISPR/Cas14a-synergized wood-based platform (CWP) is designed here to detect aflatoxin B1 (AFB1) at 0.67 fmol/L with high sensitivity and no preamplification. Once perform an assay, AFB1 is optical identified through an aptamer competition process, which triggers the trans-cleavage activity of Cas14a, resulting significant color changes on the wood surface once introducing chromogenic substrates. This method can be conveniently extended to the detection of disease-related protein biomarkers with low detection thresholds.