PolyA-Tail-Mediated Assembly of a Gold Nanoparticle-Based Turn-on Fluorescent Aptasensor for Ultrasensitive Detection of Perfluorooctanoic acid
Abstract
A turn-on fluorescent aptasensor for PFOA is developed using a polyA-tailed aptamer anchored on gold nanoparticles and a fluorescently labeled cDNA. The displacement of cDNA by PFOA restores fluorescence, enabling ultrasensitive detection with a 1.3 nmol L-1 limit, demonstrated by satisfactory recovery in spiked fish samples.
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