Quantitative determination of maslinic acid and oleanolic acid in olive pomace using high-speed shear extraction and liquid chromatography

Abstract

Olive pomace contains abundant maslinic acid and oleanolic acid, maslinic acid and oleanolic acid are pentacyclic triterpenoid compounds with excellent biological activity. In this study, a method combining high-speed shear extraction and HPLC analysis was developed to determine the content of maslinic acid and oleanolic acid in olive pomace. Single-factor experiments were conducted to optimize the high-speed shear extraction conditions, including rotation speed, liquid-to-material ratio, processing time, and extraction solvent. The optimized extraction conditions were as follows: methanol as the extraction solvent, rotation speed in the range of 8000-12000 rpm, processing time of 2 minutes, and liquid-to-material ratio of 0.5 (mL/50 mg). The recovery rates of the HPLC analysis method ranged from 98.34% to 109.25%, with a relative (RSD) of less than 3.01%; LOD were 0.0035 µg/mL and 0.0038 μg/mL, and the LOQ were 0.012 µg/mL and 0.014 µg/mL; the intra-day and inter-day precisions were 2.07%, 2.95% and 2.9%, 2.85%, respectively. This method is simple to operate and enables rapid and efficient extraction of target components from olive pomace at room temperature, making it suitable for the quantitative determination of trace components in olive pomace.