A Novel Platform using a Regenerable SDS-Functionalized Membrane with AF4-DAD-MALS for Online Enrichment and Detection of Polydisperse Nanoplastics in Biological Samples

Abstract

In view of the significance and urgency of elucidating the internal exposure toxicity and transport mechanisms of nanoplastics (NPl) in organisms for assessing their health risks, we developed a novel analytical method that integrates online enrichment via a sodium dodecyl sulfate (SDS)-functionalized regenerated cellulose membrane (RCM) with asymmetric flow field-flow fractionation coupled to a diode array detector and multi-angle light scattering (AF4-DAD-MALS) to quantify 60-500 nm NPl in organisms. This method boasts high accuracy (recovery: 89-103%), precision (RSD<5.2%), sensitivity (LOD: 0.5-2.0 μg/L) and reusability. The method was applied to the blood metabolism study of polydisperse NPl, results showed larger particles exhibited prolonged retention.