Chaperone polymer-enhanced microRNA sensing on a surface-functionalised power-free microchip

Ryo Ishihara; Kotomi Katori; Manaya Ogawa; Mutsumi Hosono; Yingfan Mu; Ren Ogata; Hinako Yokohari; Rin Hirose; Eri Shimura; Takeshi Baba; Naoko Yoshida; Naoki Yoshida; Atsushi Maruyama

doi:10.1039/D6AN00188B

Chaperone polymer-enhanced microRNA sensing on a surface-functionalised power-free microchip

Ryo Ishihara,

*^a Kotomi Katori,^b Manaya Ogawa,^c Mutsumi Hosono,^c Yingfan Mu,^d Ren Ogata,^d Hinako Yokohari,^b Rin Hirose,^a Eri Shimura,

^a Takeshi Baba,^a Naoko Yoshida,

^a Naoki Yoshida^e and Atsushi Maruyama

*^ef

Author affiliations

* Corresponding authors

^a Faculty of Medicine, Juntendo University, Chiba 270-1695, Japan
E-mail: ishihara@juntendo.ac.jp

^b Faculty of Health and Sports Science, Juntendo University, Chiba 270-1695, Japan

^c Department of Applied Chemistry, Chiba Institute of Technology, Chiba 275-0016, Japan

^d Faculty of International Liberal Arts, Juntendo University, Tokyo 113-8421, Japan

^e Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8503, Japan
E-mail: amrym@toyaku.ac.jp

^f Department of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji 192-0392, Japan

Abstract

Artificial chaperone polymer-enhanced hybridisation enabled the sensitive and specific detection of hsa-miR-500a-3p, a liquid biopsy biomarker, from a 1.0 µL sample within 15 minutes using a portable surface-functionalised power-free microchip. This chaperone polymer improved the sensitivity by over three orders of magnitude. This is the first demonstration of chaperone polymer-accelerated hybridisation between immobilised DNA and target microRNA.

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Chaperone polymer-enhanced microRNA sensing on a surface-functionalised power-free microchip

Abstract

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