Chaperone Polymer-Enhanced MicroRNA Sensing on a Surface-Functionalised Power-Free Microchip

Abstract

Artificial Chaperone polymer-enhanced hybridisation enabled the sensitive and specific detection of hsa-miR-500a-3p, a liquid biopsy biomarker, from a 1.0 µL sample within 15 minutes using a portable surface-functionalised power-free microchip. This chaperone polymer improved the sensitivity by over three orders of magnitude. This is the first demonstration of chaperone polymer-accelerated hybridisation between immobilised DNA and target microRNA.