Volume 4, 2025

Ultrasensitive electrochemical detection of methotrexate in biological fluids using NiMn2O4/CNT nanocomposite-modified electrode

Abstract

Methotrexate (MTX) is a widely used chemotherapeutic drug with a narrow therapeutic index, making its precise monitoring crucial for effective treatment and minimizing side effects. This study focuses on the development of a clinically applicable NiMn2O4/CNT nanocomposite-modified glassy carbon electrode (NiMn2O4/CNT-GCE) for the sensitive and selective electrochemical detection of MTX. The NiMn2O4 nanomaterial was synthesized via a co-precipitation method followed by calcination, and its composite with CNTs was optimized to enhance electrochemical performance. The sensor demonstrated a detection limit as low as 0.627 nM and a broad linear detection range (0.05–3 μM), attributed to the synergistic effects of NiMn2O4 and CNTs that enhance electron transfer and active site availability. Moreover, the NiMn2O4/CNT-GCE was successfully applied to detect MTX in spiked serum and urine samples, achieving recovery rates of 96–99% with relative standard deviations below 3.5%. Its minimal interference with common metabolites and excellent stability makes it ideal for therapeutic drug monitoring. This work underscores the potential of NiMn2O4/CNT as a promising platform for real-time clinical diagnostics and advanced electrochemical sensing applications.

Graphical abstract: Ultrasensitive electrochemical detection of methotrexate in biological fluids using NiMn2O4/CNT nanocomposite-modified electrode

Supplementary files

Article information

Article type
Paper
Submitted
09 May 2025
Accepted
10 Jul 2025
First published
18 Jul 2025
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2025,4, 803-814

Ultrasensitive electrochemical detection of methotrexate in biological fluids using NiMn2O4/CNT nanocomposite-modified electrode

N. Abbas and T. H. Kim, Sens. Diagn., 2025, 4, 803 DOI: 10.1039/D5SD00064E

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