β-Lactamase cleavable antimicrobial peptide–drug conjugates

Abstract

Antimicrobial resistance attracts a considerable amount of attention as it threatens the efficiency of current antibacterial treatments. Besides a more considerate use of current antibiotics to slow down the spread of antimicrobial resistance, there is ample need for new therapeutic avenues to treat already resistant strains. Here, we describe the use of a cleavable peptide–drug conjugate to target bacteria with diverse resistance strategies. The conjugate consists of three main components: a β-lactamase cleavable linker, a positively charged stapled antimicrobial peptide, and an antibiotic. The linker ensures selective cleavage and provides the prospect of lowering systemic toxicity of the conjugate. The positively charged peptide targets the negatively charged bacterial membrane, and stapling pre-organises it in a helical structure. Finally, the drug provides another, distinct mode of action to the peptide, which should overall reduce the development of resistance. A series of peptides was prepared and the most promising one was then developed into a stapled conjugate. The factors affecting the activity of this conjugate were investigated, proving cleavage by β-lactamase and superior potency compared to the non-cleavable control, as shown by its minimal inhibitory concentrations.

Graphical abstract: β-Lactamase cleavable antimicrobial peptide–drug conjugates

Supplementary files

Article information

Article type
Edge Article
Submitted
20 Aug 2025
Accepted
11 Sep 2025
First published
19 Sep 2025
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2025, Advance Article

β-Lactamase cleavable antimicrobial peptide–drug conjugates

T. Deingruber, J. S. Gaynord, B. H. Gan, K. A. Kostadinova, T. J. O'Brien, Y. S. Tan, J. S. Parker, T. A. Hunt, J. S. Carroll, M. Welch and D. R. Spring, Chem. Sci., 2025, Advance Article , DOI: 10.1039/D5SC06369H

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