A dual-excitation ratiometric NIR-II fluorescent nanoplatform enables high contrast in vivo imaging

Abstract

Ratiometric fluorescent probes in the second near-infrared window (NIR-II) with a self-calibration function are sought after for reliable imaging of physiological and pathological processes. Nevertheless, current ratiometric NIR-II fluorescent probes usually show severe spectral overlap in the emission channel, resulting in inevitable sacrifice of the emission intensity of the probe and compromised imaging quality in the NIR-II region. To address these challenges, we developed a novel dual-excitation ratiometric NIR-II fluorescence nanoplatform (DERF-NP), in which the intensity ratio of the same full-wavelength emission from 1000 to 1700 nm under two non-overlapping monochrome excitations with distinct responses is conventionally defined as the quantification parameter. As a proof of concept, DERF-NO, our test case of a ratiometric NIR-II nanoprobe for NO imaging with high sensitivity and quality, was developed based on an energy transfer strategy, which showed increased emission with excitation at 660 nm and constant emission with excitation at 808 nm upon activation of NO. In vivo ratiometric NIR-II fluorescence imaging with DERF-NO successfully tracked macrophage polarization and lymphatic metastasis, suggesting the extensive distribution and critical role of macrophages in tumorigenesis and progression. This dual-excitation ratiometric imaging strategy may provide a novel approach for designing ratiometric NIR-II fluorescent probes and has great application potential for intravital imaging analysis.

Graphical abstract: A dual-excitation ratiometric NIR-II fluorescent nanoplatform enables high contrast in vivo imaging

Supplementary files

Article information

Article type
Edge Article
Submitted
11 Apr 2025
Accepted
23 Jun 2025
First published
24 Jun 2025
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2025, Advance Article

A dual-excitation ratiometric NIR-II fluorescent nanoplatform enables high contrast in vivo imaging

Y. Liu, L. Teng, B. Zhang, X. Zhang and G. Song, Chem. Sci., 2025, Advance Article , DOI: 10.1039/D5SC02705E

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