Development of a point-of-care-testing platform: localized surface plasmon resonance biosensor for rapid ABO/Rh blood typing

Abstract

Point-of-care testing (POCT) devices are crucial for clinical diagnosis. Presently, clinical biochemical testing heavily relies on advanced analytical equipment, often necessitating bulky machinery and extended assay periods. This study presents a localized surface plasmon resonance biosensor-based POCT platform that integrates microfluidic methods for rapid and cost-effective detection of ABO/Rh blood types. The autonomous system enables high-throughput detection of ABO/Rh blood types with high specificity and sensitivity within 20 minutes using a spectrum based readout. Additionally, we have integrated eight internal microfluidic channels into the portable system to facilitate a high-throughput detection solution. Moreover, the incorporation of microfluidic channels minimizes sample cross-contamination, thereby enhancing detection precision. We believe this rapid, convenient, and cost-effective blood typing platform can serve as a valuable tool in clinical POCT applications.