Issue 22, 2025

Synthesis and characterization of novel N-(2-(pyrazin-2-yl-oxy)ethyl)-4-(trifluoromethoxy)benzamide scaffolds, and biological evaluation and molecular docking studies

Abstract

A new series of biologically potent N-(2-(6-substituted-1H-pyrazin-2-yloxy)ethyl)-4-(trifluoromethoxy)benzamide scaffolds was synthesized, and their structures were confirmed by 1H NMR, 13C NMR, and mass spectrometry. All the synthesized molecules were tested against antibacterial activity against various pathogenic microorganisms and exhibited remarkable activity. Compounds 12a and 13a exhibited good antibacterial activity against pathogenic cell lines, Staphylococcus aureus and Escherichia coli. Additionally, synthesized molecules 12a and 13a were screened for anticancer activity against the A549 (lung cancer) cell line. These compounds displayed excellent anticancer activity with IC50 values of 19 + 0.50 μM, 17 ± 0.5 μM, A549 (lung cancer). Molecular docking studies results were well supported by strong intercalative interactions of the synthesized compounds with target proteins.

Graphical abstract: Synthesis and characterization of novel N-(2-(pyrazin-2-yl-oxy)ethyl)-4-(trifluoromethoxy)benzamide scaffolds, and biological evaluation and molecular docking studies

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Article information

Article type
Paper
Submitted
06 Feb 2025
Accepted
15 May 2025
First published
23 May 2025
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2025,15, 17290-17301

Synthesis and characterization of novel N-(2-(pyrazin-2-yl-oxy)ethyl)-4-(trifluoromethoxy)benzamide scaffolds, and biological evaluation and molecular docking studies

V. K. Prasad. B, G. V. Haritha, K. Shireesha, K. S. Jella, D. Ravi and A. Ramesh, RSC Adv., 2025, 15, 17290 DOI: 10.1039/D5RA00879D

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