Injectable carboxymethyl chitosan/oxidized dextran hydrogels containing zoledronic acid modified strontium hydroxyapatite nanoparticles

Abstract

Nanocomposite hydrogels have potential in bone regeneration due to the inorganic and polymeric material content. In this study, new types of nanocomposite hydrogels composed of zoledronic acid/strontium hydroxyapatite nanoparticles and carboxymethyl chitosan/oxidized dextran (CMC/OD) hydrogels were reported. Pure hydroxyapatite, 5%, 10% and 15% (w/w) strontium-substituted strontium hydroxyapatite nanoparticles were produced and then modified with zoledronic acid at ratios of 5% to 7.5% (w/w). These modified structures were then incorporated into CMC/OD hydrogels. Zoledronic acid modified strontium hydroxyapatite nanoparticles were characterized using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS) and X-ray Diffraction (XRD). CMC/OD structures were investigated using Fourier Transform Infrared Analysis (FTIR), Scanning Electron Microscopy (SEM). The physical properties of the hydrogels were determined via degradation behavior and rheological measurements. Cell–material interactions were investigated in vitro. The results showed that the incorporation of hydroxyapatite nanoparticles into CMC would significantly improve the rheological properties. The addition of strontium to hydroxyapatite nanoparticles significantly enhanced cell proliferation. In addition, a significant increase in alkaline phosphatase (ALP) and calcium deposition was observed with the addition of zoledronic acid. In conclusion, the nanocomposite hydrogels of CMC/OD containing zoledronic acid modified strontium hydroxyapatite demonstrate potential for orthopedic and craniofacial applications due to their superior properties, including the ability to be easily injected into targeted areas, potent antibacterial activity that helps prevent infections and remarkable self-healing capabilities that promote tissue regeneration and repair.