Immunocytochemistry response of a hybrid (chitosan-g-glycidyl methacrylate)-xanthan scaffold for cell proliferation

Abstract

In the present work, chitosan-glycidyl methacrylate-xanthan ((CTS-g-GMA)-X) hydrogel was successfully synthesized by the chemical reaction of a co-polymer of chitosan-g-glycidyl methacrylate (CTS-g-GMA), under different stoichiometric molar ratios from 1 : 1 to 1 : 4, and xanthan (X). (CTS-g-GMA)-X was synthesized by two methods to obtain a hydrogel: an aqueous acid media followed by neutralization and a neutral aqueous media, with an extended reaction time. Human epidermal keratinocytes (HEK) and nerve cells (neuroblastoma × glioma hybrid cell NG108-15) were sown over the hydrogels, and their vitality was determined using a calcein stain. The viability for keratinocytes and nerve cells was quantified using a DNA (proliferation) assay at several intervals. Additionally, immunocytochemistry, detecting E-cadherin, fibronectin, and laminin proteins in HEK, was performed to discard possible diseases caused by (CTS-g-GMA)-X hydrogels. The (CTS-GMA) shows greater results compared with the positive control (glass) and the pure chitosan; all polymers show similar satisfactory behaviors between them.