Charges on a suspended silicon nitride membrane under high energy electron beam

Abstract

Thin silicon nitride (SiN_x) membranes are widely used in gas and liquid phase transmission electron microscopy (TEM) and as phase plates to enhance imaging contrast. As a dielectric material, SiN_x contains trap sites for both positive and negative charges, which can be manipulated by high-energy electron irradiation, external biasing, or light exposure. Charge accumulation on the membrane can significantly affect in situ TEM processes, including chemical and electrochemical reactions, nanoparticle dynamics, and catalytic activity, or introduce unwanted phase shifts when used as a phase plate. In this study, charge accumulation on suspended SiN_x membranes was investigated using off-axis electron holography combined with model-free phase analysis, supported by custom finite element analysis (FEA) simulations. An average residual positive charge density of approximately 2.8* 10^-4Cm^-2 was measured. Localized and stable regions of both positive and negative charges were identified on the membrane. The global positive and localized positive/negative charges give rise to strong electric fields and electroosmotic slip velocities at the membrane surface, and sufficient to induce non-Brownian particle behavior and directional fluid flow, offering a physical explanation for previously observed anomalies in particle dynamics, nucleation, and growth during gas and liquid phase TEM experiments. These results provide a benchmark for understanding charge behavior at SiN_x interfaces in gas and liquid phase TEM. Furthermore, the FEA simulations establish a framework for future investigations into charge distribution, electrostatic potentials, and electrical double layers at solid-liquid interfaces, particularly in complex geometries and chemically dynamic environ