A sustainable enzyme-laden hydrogel via heterodimeric α-helices induced self-assembly

Abstract

Herein, we developed a mild, sustainable, and biocompatible method for enzyme immobilization. This method exploits salt bridge interactions between E3/K3 heterodimers to immobilize E3-functionalized lipase into collagen hydrogels modified with E3/K3 motifs. Benefiting from high specificity and improved organic solvent tolerance, as well as the high biocapacity of collagen-hydrogels, this enzyme-laden immobilization method holds promising potential for applications in the medical industry.