From the journal:

Nanoscale Horizons

FluMag-SELEX derived specific individual aptamers can fluorescently label the ageing immanent protein-crosslinker glucosepane in diabetic mouse tissues

Runliu Li,a   Bastian Draphoen,b   Mika Lindén, ORCID logo b   Karmveer Singh,c   Karin Scharffetter-Kochanek,c   Andreas Stürmer, ORCID logo d   Frank Rosenau ORCID logo *a  and  Ann-Kathrin Kissmann ORCID logo *ae  

* Corresponding authors

a Institute of Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany
E-mail: ann-kathrin.kissmann@uni-ulm.de

b Institute of Inorganic Chemistry II, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany

c Department of Dermatology and Allergic Diseases, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany

d T-Technologies Grundlagenforschung e.U., Sonntagberg 10, 4550 Kremsmünster, Austria

e Danube Private University, Steiner Landstraße 124, Krems an der Donau 3500, Austria

Abstract

Nowadays, aptamers have transitioned into valuable antibody alternatives. We present the first anti-glucosepane aptamer targeting a key glycation product linked to aging and diabetes. Glu3, with high specificity and affinity, enabled the first-ever direct, fluorescence-based histological staining of glucosepane in murine tissue, distinguishing diabetic samples from wild-type samples without secondary reagents.

Graphical abstract: FluMag-SELEX derived specific individual aptamers can fluorescently label the ageing immanent protein-crosslinker glucosepane in diabetic mouse tissues

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Article information

DOI
https://doi.org/10.1039/D5NH00263J
Article type
Communication
Submitted
22 Apr 2025
Accepted
06 Aug 2025
First published
14 Aug 2025
This article is Open Access
Creative Commons BY license

Nanoscale Horiz., 2025, Advance Article

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FluMag-SELEX derived specific individual aptamers can fluorescently label the ageing immanent protein-crosslinker glucosepane in diabetic mouse tissues

R. Li, B. Draphoen, M. Lindén, K. Singh, K. Scharffetter-Kochanek, A. Stürmer, F. Rosenau and A. Kissmann, Nanoscale Horiz., 2025, Advance Article , DOI: 10.1039/D5NH00263J

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