MiniSOG as a Biodegradable Heterogeneous Photocatalyst for Coupled Redox Biotransformations

Abstract

Visible‐light photocatalysis has emerged as a powerful strategy to drive chemical reactions under mild conditions, often in tandem with biocatalysts to achieve one‐pot cascades. In this work, we demonstrate that the photocatalytic flavoprotein miniSOG, originally engineered as a genetically encoded singlet oxygen generator, can be repurposed as a heterogeneous photocatalyst. By immobilizing miniSOG as cross‐linked enzyme aggregates (CLEAs), we enable controlled in situ production of hydrogen peroxide (H₂O₂) and NADH regeneration, facilitating both oxidative and reductive biotransformations.